Characteristics of Salmonella Recovered From Stools of Children Enrolled in the Global Enteric Multicenter Study

Details

• Alternative Title:
  Clin Infect Dis
• Personal Author:
  Kasumba, Irene N ; Pulford, Caisey V ; Perez-Sepulveda, Blanca M ; Sen, Sunil ; Sayed, Nurulla ; Permala-Booth, Jasnehta ; Livio, Sofie ; Heavens, Darren ; Low, Ross ; Hall, Neil ; Roose, Anna ; Powell, Helen ; Farag, Tamer ; Panchalingham, Sandra ; Berkeley, Lynette ; Nasrin, Dilruba ; Blackwelder, William C ; Wu, Yukun ; Tamboura, Boubou ; Sanogo, Doh ; Onwuchekwa, Uma ; Sow, Samba O ; Ochieng, John B ; Omore, Richard ; Oundo, Joseph O ; Breiman, Robert F ; Mintz, Eric D ; O’Reilly, Ciara E ; Antonio, Martin ; Saha, Debasish ; Hossain, M Jahangir ; Mandomando, Inacio ; Bassat, Quique ; Alonso, Pedro L ; Ramamurthy, T ; Sur, Dipika ; Qureshi, Shahida ; Zaidi, Anita K M ; Hossain, Anowar ; Faruque, Abu S G ; Nataro, James P ; Kotloff, Karen L ; Levine, Myron M ; Hinton, Jay C D ; Tennant, Sharon M
• Description:
  Background
  
  The Global Enteric Multicenter Study (GEMS) determined the etiologic agents of moderate-to-severe diarrhea (MSD) in children under 5 years old in Africa and Asia. Here, we describe the prevalence and antimicrobial susceptibility of nontyphoidal Salmonella (NTS) serovars in GEMS and examine the phylogenetics of Salmonella Typhimurium ST313 isolates.
  
  Methods
  
  Salmonella isolated from children with MSD or diarrhea-free controls were identified by classical clinical microbiology and serotyped using antisera and/or whole-genome sequence data. We evaluated antimicrobial susceptibility using the Kirby-Bauer disk-diffusion method. Salmonella Typhimurium sequence types were determined using multi-locus sequence typing, and whole-genome sequencing was performed to assess the phylogeny of ST313.
  
  Results
  
  Of 370 Salmonella-positive individuals, 190 (51.4%) were MSD cases and 180 (48.6%) were diarrhea-free controls. The most frequent Salmonella serovars identified were Salmonella Typhimurium, serogroup O:8 (C2-C3), serogroup O:6,7 (C1), Salmonella Paratyphi B Java, and serogroup O:4 (B). The prevalence of NTS was low but similar across sites, regardless of age, and was similar among both cases and controls except in Kenya, where Salmonella Typhimurium was more commonly associated with cases than controls. Phylogenetic analysis showed that these Salmonella Typhimurium isolates, all ST313, were highly genetically related to isolates from controls. Generally, Salmonella isolates from Asia were resistant to ciprofloxacin and ceftriaxone, but African isolates were susceptible to these antibiotics.
  
  Conclusions
  
  Our data confirm that NTS is prevalent, albeit at low levels, in Africa and South Asia. Our findings provide further evidence that multidrug-resistant Salmonella Typhimurium ST313 can be carried asymptomatically by humans in sub-Saharan Africa.
  
  
• Subjects:
  Anti-Bacterial Agents Child Child, Preschool Humans Multilocus Sequence Typing Phylogeny Salmonella Infections Salmonella Typhimurium
• Source:
  Clin Infect Dis. 2021; 73(4):631-641
• Pubmed ID:
  33493332
• Pubmed Central ID:
  PMC8366818
• Document Type:
  Journal Article
• Funding:
  WT_/Wellcome TrustUnited Kingdom/ ; 38874/Bill and Melinda Gates Foundation/ ; Global Challenges Research Fund/ ; BBS/OS/GC/000009D/Earlham Institute/ ; BB/CCG1720/1/Core Strategic Program of the Earlham Institute/ ; BB/CCG1720/1/BB_/Biotechnology and Biological Sciences Research CouncilUnited Kingdom/ ; 106914/Z/15/Z/WT_/Wellcome TrustUnited Kingdom/
• Place as Subject:
  Kenya
• Volume:
  73
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:31508b3d7c00d58e88013124a4522267a4126beeddfc32c9fec9c128c8c6fc67
• Download URL:
  https://stacks.cdc.gov/view/cdc/111644/cdc_111644_DS1.pdf
• File Type:
  [PDF - 2.80 MB ]