Details:

Alternative Title:
Parasit Vectors
Personal Author:
Monte, Tainá C. C. ; Simões, Raquel O. ; Oliveira, Ana Paula M. ; Novaes, Clodoaldo F. ; Thiengo, Silvana C. ; ... More +
Monte, Tainá C. C. ; Simões, Raquel O. ; Oliveira, Ana Paula M. ; Novaes, Clodoaldo F. ; Thiengo, Silvana C. ; Silva, Alexandre J. ; Estrela, Pedro C. ; Júnior, Arnaldo Maldonado Less -
Description:
Background

The rat lungworm Angiostrongylus cantonensis can cause eosinophilic meningoencephalitis in humans. This nematode’s main definitive hosts are rodents and its intermediate hosts are snails. This parasite was first described in China and currently is dispersed across several Pacific islands, Asia, Australia, Africa, some Caribbean islands and most recently in the Americas. Here, we report the genetic variability among A. cantonensis isolates from different geographical locations in Brazil using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences.

Methods

The isolates of A. cantonensis were obtained from distinct geographical locations of Brazil. Genomic DNAs were extracted, amplified by polymerase reaction, purified and sequenced. A partial sequence of COI gene was determined to assess their phylogenetic relationship.

Results

The sequences of A. cantonensis were monophyletic. We identified a distinct clade that included all isolates of A. cantonensis from Brazil and Asia based on eight distinct haplotypes (ac1, ac2, ac3, ac4, ac5, ac6, ac7 and ac8) from a previous study. Interestingly, the Brazilian haplotype ac5 is clustered with isolates from Japan, and the Brazilian haplotype ac8 from Rio de Janeiro, São Paulo, Pará and Pernambuco states formed a distinct clade. There is a divergent Brazilian haplotype, which we named ac9, closely related to Chinese haplotype ac6 and Japanese haplotype ac7.

Conclusion

The genetic variation observed among Brazilian isolates supports the hypothesis that the appearance of A. cantonensis in Brazil is likely a result of multiple introductions of parasite-carrying rats, transported on ships due to active commerce with Africa and Asia during the European colonization period. The rapid spread of the intermediate host, Achatina fulica, also seems to have contributed to the dispersion of this parasite and the infection of the definitive host in different Brazilian regions.


Subjects:
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Angiostrongylus Cantonensis Animals Cluster Analysis Electron Transport Complex IV Gastropoda Genetic Variation Mitochondrial Proteins Molecular Sequence Data Phylogeny Rats Research Sequence Analysis, DNA
Source:
Parasit Vectors. 2012; 5:248.
Document Type:
Journal Article
Place as Subject:
Brazil
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:b964fd98a44e55baa6b4a1a8b4c4838a284a29b58a836a5761ea8ca55bfcb017
Download URL:
https://stacks.cdc.gov/view/cdc/11051/cdc_11051_DS1.pdf
File Type:

Supporting Files

• 	license.txt	txt
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  	1756-3305-5-248-1.jpg	jpeg
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  	1756-3305-5-248-S1.pdf	pdf
  	1756-3305-5-248.nxml	txt

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