Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Soumana, Illiassou Hamidou ; Linz, Bodo ; Dewan, Kalyan K. ; Sarr, Demba ; Gestal, Monica C. ; Howard, Laura K. ; Caulfield, Amanda D. ; Rada, Balázs ; Harvill, Eric T.
• Description:
  Conventional pertussis animal models deliver hundreds of thousands of Bordetella pertussis bacteria deep into the lungs, rapidly inducing severe pneumonic pathology and a robust immune response. However, human infections usually begin with colonization and growth in the upper respiratory tract. We inoculated only the nasopharynx of mice to explore the course of infection in a more natural exposure model. Nasopharyngeal colonization resulted in robust growth in the upper respiratory tract but elicited little immune response, enabling prolonged and persistent infection. Immunization with human acellular pertussis vaccine, which prevents severe lung infections in the conventional pneumonic infection model, had little effect on nasopharyngeal colonization. Our infection model revealed that B. pertussis can efficiently colonize the mouse nasopharynx, grow and spread within and between respiratory organs, evade robust host immunity, and persist for months. This experimental approach can measure aspects of the infection processes not observed in the conventional pneumonic infection model.
• Subjects:
  Animals Bordetella Infections Immune Evasion Mice Nasopharynx Pertussis Vaccine Whooping Cough
• Source:
  Emerg Infect Dis. 27(8):2107-2116
• Pubmed ID:
  34286682
• Pubmed Central ID:
  PMC8314809
• Document Type:
  Journal Article
• Funding:
  R01 GM113681/GM/NIGMS NIH HHSUnited States/ ; R21 AI142678/AI/NIAID NIH HHSUnited States/ ; NH/NIH HHSUnited States/
• Volume:
  27
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:6eff6f8352dbfd5db920a0662672669c869acc3954645f366ea26dc994e9f231
• Download URL:
  https://stacks.cdc.gov/view/cdc/108780/cdc_108780_DS1.pdf
• File Type:
  [PDF - 2.58 MB ]