Experimental Infection of Horses with West Nile virus

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Bunning, Michel L. ; Bowen, Richard A. ; Cropp, Bruce C. ; Sullivan, Kevin G. ; Davis, Brent S. ; Komar, Nicholas ; Godsey, Marvin ; Baker, Dale ; Hettler, Danielle L. ; Holmes, Derek A. ; Biggerstaff, Brad J. ; Mitchell, Carl J.
• Description:
  A total of 12 horses of different breeds and ages were infected with West Nile virus (WNV) via the bites of infected Aedes albopictus mosquitoes. Half the horses were infected with a viral isolate from the brain of a horse (BC787), and half were infected with an isolate from crow brain (NY99-6625); both were NY99 isolates. Postinfection, uninfected female Ae. albopictus fed on eight of the infected horses. In the first trial, Nt antibody titers reached >1:320, 1:20, 1:160, and 1:80 for horses 1 to 4, respectively. In the second trial, the seven horses with subclinical infections developed Nt antibody titers >1:10 between days 7 and 11 post infection. The highest viremia level in horses fed upon by the recipient mosquitoes was approximately 460 Vero cell PFU/mL. All mosquitoes that fed upon viremic horses were negative for the virus. Horses infected with the NY99 strain of WNV develop low viremia levels of short duration; therefore, infected horses are unlikely to serve as important amplifying hosts for WNV in nature.
• Subjects:
  Aedes Aedes Albopictus Animals Antibodies, Viral Bird Diseases Brain Cercopithecus Aethiops Female Horse Diseases Horses Insect Vectors Male Research Saliva Songbirds Vero Cells Viremia West Nile Fever

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• Source:
  Emerg Infect Dis. 8(4):380-386.
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:8842621e61aa27bd34bc947ffac25bb9d5a5ff3dca948ed706e3133379521989
• Download URL:
  https://stacks.cdc.gov/view/cdc/10791/cdc_10791_DS1.pdf
• File Type:
  [PDF - 326.38 KB ]