Conventional and High-Sensitivity Malaria Rapid Diagnostic Test Performance in 2 Transmission Settings: Haiti 2017

Details

• Alternative Title:
  J Infect Dis
• Personal Author:
  Rogier, Eric ; Hamre, Karen E. S. ; Joseph, Vena ; Plucinski, Mateusz M. ; Presume, Jacquelin ; Romilus, Ithamare ; Mondelus, Gina ; Elisme, Tamara ; van den Hoogen, Lotus ; Lemoine, Jean Frantz ; Drakeley, Chris ; Ashton, Ruth A. ; Chang, Michelle A. ; Existe, Alexandre ; Boncy, Jacques ; Stresman, Gillian ; Druetz, Thomas ; Eisele, Thomas P.
• Description:
  Accurate malaria diagnosis is foundational for control and elimination, and Haiti relies on histidine-rich protein 2 (HRP2)-based rapid diagnostic tests (RDTs) identifying Plasmodium falciparum in clinical and community settings. In 2017, 1 household and 2 easy-access group surveys tested all participants (N = 32 506) by conventional and high-sensitivity RDTs. A subset of blood samples (n = 1154) was laboratory tested for HRP2 by bead-based immunoassay and for P. falciparum 18S rDNA by photo-induced electron transfer polymerase chain reaction. Both RDT types detected low concentrations of HRP2 with sensitivity estimates between 2.6 ng/mL and 14.6 ng/mL. Compared to the predicate HRP2 laboratory assay, RDT sensitivity ranged from 86.3% to 96.0% between tests and settings, and specificity from 90.0% to 99.6%. In the household survey, the high-sensitivity RDT provided a significantly higher number of positive tests, but this represented a very small proportion (<0.2%) of all participants. These data show that a high-sensitivity RDT may have limited utility in a malaria elimination setting like Haiti.
• Subjects:
  Adolescent Antigens, Protozoan Child Child, Preschool Diagnostic Tests, Routine DNA, Protozoan DNA, Ribosomal Enzyme-Linked Immunosorbent Assay Female Humans Infant Malaria, Falciparum Male Polymerase Chain Reaction Protozoan Proteins Sensitivity And Specificity

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• Source:
  J Infect Dis. 221(5):786-795
• Pubmed ID:
  31630194
• Pubmed Central ID:
  PMC8211410
• Document Type:
  Journal Article
• Funding:
  R18 CK000102/CK/NCEZID CDC HHS/United States
• Place as Subject:
  Haiti
• Volume:
  221
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:2c061da73ec8715122e6bc91112339a1dd49398a9292da6154829fab1612e575
• Download URL:
  https://stacks.cdc.gov/view/cdc/107382/cdc_107382_DS1.pdf
• File Type:
  [PDF - 1.18 MB ]