Recommendations on the measurement and the clinical use of vitamin D metabolites and vitamin D binding protein – A position paper from the IFCC Committee on bone metabolism

Details

• Alternative Title:
  Clin Chim Acta
• Personal Author:
  Makris, Konstantinos ; Bhattoa, Harjit P ; Cavalier, Etienne ; Phinney, Karen ; Sempos, Christopher T. ; Ulmer, Candice Z. ; Vasikaran, Samuel D. ; Vesper, Hubert ; Heijboer, Annemieke C.
• Description:
  Vitamin D, an important hormone with a central role in calcium and phosphate homeostasis, is required for bone and muscle development as well as preservation of musculoskeletal function. The most abundant vitamin D metabolite is 25-hydroxyvitamin D [25(OH)D], which is currently considered the best marker to evaluate overall vitamin D status. 25(OH)D is therefore the most commonly measured metabolite in clinical practice. However, several other metabolites, although not broadly measured, are useful in certain clinical situations. Vitamin D and all its metabolites are circulating in blood bound to vitamin D binding protein, (VDBP). This highly polymorphic protein is not only the major transport protein which, along with albumin, binds over 99% of the circulating vitamin D metabolites, but also participates in the transport of the 25(OH)D into the cell via a megalin/cubilin complex. The accurate measurement of 25(OH)D has proved a difficult task. Although a reference method and standardization program are available for 25(OH)D, the other vitamin D metabolites still lack this. Interpretation of results, creation of clinical supplementation, and generation of therapeutic guidelines require not only accurate measurements of vitamin D metabolites, but also the accurate measurements of several other "molecules" related with bone metabolism. IFCC understood this priority and a committee has been established with the task to support and continue the standardization processes of vitamin D metabolites along with other bone-related biomarkers. In this review, we present the position of this IFCC Committee on Bone Metabolism on the latest developments concerning the measurement and standardization of vitamin D metabolites and its binding protein, as well as clinical indications for their measurement and interpretation of the results.
• Subjects:
  Biomarkers Bone And Bones Calcifediol Humans Vitamin D Vitamin D-Binding Protein
• Keywords:
  1α,25-dihydroxyvitamin D 24,25-dihydroxyvitamin D 25-hydroxyvitamin D Immunoassays Liquid Chromatography Mass Spectrometry Standardization Vitamin D Binding Protein Vitamin D Standardization Program
• Source:
  Clin Chim Acta. 517:171-197
• Pubmed ID:
  33713690
• Pubmed Central ID:
  PMC8080555
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  517
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:15c1d275641127636a2afdcfa3b388f91cd84189425a137dabc0b6dbdb8b72e7
• Download URL:
  https://stacks.cdc.gov/view/cdc/105698/cdc_105698_DS1.pdf
• File Type:
  [PDF - 1.36 MB ]