Trisaminohexyl isocyanurate, a urinary biomarker of HDI isocyanurate exposure

Details

• Alternative Title:
  J Chromatogr B Analyt Technol Biomed Life Sci
• Personal Author:
  Robbins, Zachary ; Bodnar, Wanda ; Zhang, Zhenfa ; Gold, Avram ; Nylander-French, Leena A.
• Description:
  Biological monitoring of occupational exposure to 1,6-hexamethylene diisocyanate (HDI)-containing spray-paints is limited to analysis of metabolites of HDI monomer although polymeric HDI isocyanurate constitutes the predominant inhalation and skin exposure for workers in the automotive paint industry. A novel method using nanoflow ultra-performance liquid chromatography coupled to nano-electrospray ionization tandem mass spectrometry (nano-UPLC-ESI-MS/MS) was developed to quantify trisaminohexyl isocyanurate (TAHI), a hydrolysis product of HDI isocyanurate, in the urine of spray-painters. Analytical and internal standards were synthesized in-house and weighted linear regression calibration curves were generated using spiked control urine from non-exposed persons (0.06-7.98 μg/L; N = 13; w = x|; r = 0.998). Urine samples collected from 15 exposed workers (N = 111) were subjected to acid hydrolysis and extracted with dichloromethane, then derivatized with acetic anhydride. The derivatized product, trisacetamidohexyl isocyanurate (TAAHI), was analyzed using nano-UPLC-ESI-MS/MS. The protocol was sensitive and specific for analysis of TAHI in the urine of exposed workers with a method detection limit at 0.03 μg/L. TAHI was detected in 33 of 111 urine samples and in 11 of 15 workers. This biomarker for HDI isocyanurate is critical to determine the relative potency and dose-relationships between the monomer and oligomer exposure on the development of diisocyanate induced health effects in future studies.
• Subjects:
  1,6-Hexamethylene Diisocyanate Article Biomarker Biomarkers Chromatography, High Pressure Liquid Exposure Humans Isocyanate Isocyanates Isocyanurate Liquid Chromatography–mass Spectrometry Male Nanotechnology Occupational Exposure Paint Tandem Mass Spectrometry Triazines

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• Source:
  J Chromatogr B Analyt Technol Biomed Life Sci. 1076:117-129
• Pubmed ID:
  29406025
• Pubmed Central ID:
  PMC8011845
• Document Type:
  Journal Article
• Funding:
  P30 ES010126/ES/NIEHS NIH HHS/United States ; T42 OH008673/OH/NIOSH CDC HHS/United States ; R01 OH007598/OH/NIOSH CDC HHS/United States ; R01 OH010476/OH/NIOSH CDC HHS/United States ; T42OH008673/ACL/ACL HHS/United States
• Volume:
  1076
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:d3f25aec891ffd2ab0ea197a8404a872ed3aa36b1b0cd404d7163d347cc16484
• Download URL:
  https://stacks.cdc.gov/view/cdc/104816/cdc_104816_DS1.pdf
• File Type:
  [PDF - 1.32 MB ]