Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Cuevas, Luis E. ; Santos, Victor S. ; Lima, Shirley Verônica Melo Almeida ; Kontogianni, Konstantina ; Bimba, John S. ; ... More +
Cuevas, Luis E. ; Santos, Victor S. ; Lima, Shirley Verônica Melo Almeida ; Kontogianni, Konstantina ; Bimba, John S. ; Iem, Vibol ; Dominguez, Jose ; Adams, Emily ; Atienzar, Ana Cubas ; Edwards, Thomas ; Squire, S. Bertel ; Hall, Patricia J. ; Creswell, Jacob Less -
Description:
GeneXpert-based testing with Xpert MTB/RIF or Ultra assays is essential for tuberculosis diagnosis. However, testing may be affected by cartridge and staff shortages. More efficient testing strategies could help, especially during the coronavirus disease pandemic. We searched the literature to systematically review whether GeneXpert-based testing of pooled sputum samples achieves sensitivity and specificity similar to testing individual samples; this method could potentially save time and preserve the limited supply of cartridges. From 6 publications, we found 2-sample pools using Xpert MTB/RIF had 87.5% and 96.0% sensitivity (average sensitivity 94%; 95% CI 89.0%-98.0%) (2 studies). Four-sample pools averaged 91% sensitivity with Xpert MTB/RIF (2 studies) and 98% with Ultra (2 studies); combining >4 samples resulted in lower sensitivity. Two studies reported that pooling achieved 99%-100% specificity and 27%-31% in cartridge savings. Our results show that pooling may improve efficiency of GeneXpert-based testing.
Subject:
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Coronavirus Infections Cost-Benefit Analysis COVID-19 Humans Molecular Diagnostic Techniques Mycobacterium Tuberculosis SARS-CoV-2 Sensitivity And Specificity Specimen Handling Sputum Tuberculosis
Source:
Emerg Infect Dis. 27(3):719-727
Pubmed ID:
33622482
Pubmed Central ID:
PMC7920689
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:29c16ef06933ea3d78cd95e23a18a74e2e4b5b1592334c7e8023f2dd70257a1e
Download URL:
https://stacks.cdc.gov/view/cdc/104050/cdc_104050_DS1.pdf
File Type:

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