Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

Hogan, Catherine A.; Huang, ChunHong; Sahoo, Malaya K.; Wang, Hannah; Jiang, Becky; Sibai, Mamdouh; Holubar, Marisa; Mathew, Roshni; Zehnder, James; Pinsky, Benjamin A.

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Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

Supporting Files Public Domain

2021
By Hogan, Catherine A. ; Huang, ChunHong ; Sahoo, Malaya K. ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Hogan, Catherine A. ; Huang, ChunHong ; Sahoo, Malaya K. ; Wang, Hannah ; Jiang, Becky ; Sibai, Mamdouh ; Holubar, Marisa ; Mathew, Roshni ; Zehnder, James ; Pinsky, Benjamin A.
Description:

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
Subjects:

Adult Clinical Decision-Making Coronavirus Infections COVID-19 Nucleic Acid Testing Disease Transmission, Infectious Feasibility Studies Female Humans Male Middle Aged Prospective Studies Retrospective Studies RNA, Viral Virus Replication
Source:

Emerg Infect Dis. 27(2):632-635
Pubmed ID:

33496233
Pubmed Central ID:

PMC7853532
Document Type:

Journal Article
Volume:

27
Issue:

2
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:5aa6b91f0d7928150cb24b053c9d8e9d06bdc4397f93350afe159ecec628a1ea
Download URL:

https://stacks.cdc.gov/view/cdc/102483/cdc_102483_DS1.pdf
File Type:

[PDF - 1.77 MB ]

File Language:

English

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