Limited Tryptic Digestion-Isotope Dilution Mass Spectrometry (LTD-IDMS): A Reagent-Free Analytical Assay To Quantify Hemagglutinin of A(H5N1) Vaccine Material

Details

• Alternative Title:
  Anal Chem
• Personal Author:
  Cooper, Hans C. ; Xie, Yuhong ; Palladino, Giuseppe ; Barr, John R. ; Settembre, Ethan C. ; Wen, Yingxia ; Williams, Tracie L.
• Description:
  Avian influenza viruses, such as A(H5N1) and A(H7N9), are primary public health concerns due to their pandemic potential. Influenza vaccines represent the most effective response to this threat especially with timely provision. The current pandemic response timelines require a substantial period for strain-specific reference antigen and sera preparation for use with single-radial immunodiffusion (SRID), the accepted vaccine potency assay. To address this time lag, the isotope dilution mass spectrometry (IDMS) method was developed to quantify the absolute hemagglutinin (HA, the main influenza antigen) amount in the vaccine without the need for purified, inactivated, and calibrated virus reference antigens. However, an additional challenge in determining potency is to differentiate between vaccine antigens in their most potent form from other less potent, stressed antigen forms. The limited trypsin digestion (LTD) method has been developed and does not require strain-specific full-length reference antigens or antibodies; instead, stressed HA is selectively degraded, leaving the more potent form to be measured. LTD, followed by precipitation and IDMS, allows for efficient differentiation between potent and significantly less potent HA for vaccine release and potency testing across the vaccine's shelf life. In this study, we tested the LTD-IDMS assay on A(H5N1) vaccine material that had been stressed by low pH, heat, and multiple freeze-thaw cycles. The results showed that the LTD-IDMS method effectively quantified the potent HA in A(H5N1) vaccine material with results comparable to SRID. As such, it shows great promise to complement and potentially replace SRID in a pandemic when strain-specific reagents may not be readily available.
• Subjects:
  Article Hemagglutinins Humans Influenza A Virus, H5N1 Subtype Influenza A Virus, H7N9 Subtype Influenza Vaccines Mass Spectrometry
• Source:
  Anal Chem. 92(17):11879-11887
• Pubmed ID:
  32867501
• Pubmed Central ID:
  PMC7821677
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  92
• Issue:
  17
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5526fa62fd714acee07ca364199aaa602f0c4d218cc503ac4fd8fff3ab460611
• Download URL:
  https://stacks.cdc.gov/view/cdc/101074/cdc_101074_DS1.pdf
• File Type:
  [PDF - 1.02 MB ]