Reference antigen-free and antibody-free LTD-IDMS assay for influenza H7N9 vaccine in vitro potency determination

Details

• Alternative Title:
  Vaccine
• Personal Author:
  Morgenstern, Keith ; Xie, Yuhong ; Palladino, Giuseppe ; Barr, John R. ; Settembre, Ethan C. ; Williams, Tracie L. ; Wen, Yingxia
• Description:
  Influenza vaccines are the most effective intervention to prevent the substantial public health burden of seasonal and pandemic influenza. Hemagglutinin (HA), as the main antigen in inactivated influenza vaccines (IIVs), elicits functional neutralizing antibodies and largely determines IIV effectiveness. HA potency has been evaluated by single-radial immunodiffusion (SRID), the standard in vitro potency assay for IIVs, to predict vaccine immunogenicity with a correlation to protective efficacy. We previously reported that limited trypsin digestion (LTD) selectively degraded stressed HA, so that an otherwise conformationally insensitive biophysical quantification technique could specifically quantify trypsin-resistant, immunologically active HA. Here, we demonstrate that isotope dilution mass spectrometry (IDMS), a method capable of quantifying the absolute HA concentration without reference antigen use, can be further expanded by adding LTD followed with precipitation to selectively quantify the active HA. We test the LTD-IDMS assay on H7N9 vaccines stressed by low pH, raised temperature, or freeze/thaw cycles. This method, unlike SRID, has no requirement for strain-specific reference antigens or antibodies and can generate potency values that correlate with SRID. Thus, LTD-IDMS is a promising alternative in vitro potency assay for influenza vaccines to complement and potentially replace SRID in a pandemic when strain specific reagents may not be readily available.
• Subjects:
  Hemagglutinins Humans Influenza, Human Influenza A Virus, H7N9 Subtype Influenza Vaccines Isotope Labeling Mass Spectrometry
• Keywords:
  Chlorination Hemagglutinin Household Water Treatment Influenza Isotope Dilution Mass Spectrometry Limited Trypsin Digestion Potency Precipitation Safe Water Storage Single-radial Immunodiffusion Vaccine

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• Source:
  Vaccine. 36(41):6144-6151
• Pubmed ID:
  30194004
• Pubmed Central ID:
  PMC6413694
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  36
• Issue:
  41
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:bb732e902661de8138a56f15c1eff51b00cbbae64b5698ed8aa8b6d63b2d2088
• Download URL:
  https://stacks.cdc.gov/view/cdc/76924/cdc_76924_DS1.pdf
• File Type:
  [PDF - 441.71 KB ]