Biosafety in microbiological and biomedical laboratories
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Biosafety in microbiological and biomedical laboratories

  • Published Date:

    June 2020

  • Series:
    HHS publication ; no. (CDC) 300859
  • Language:
Filetype[PDF-4.35 MB]

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  • Description:
    6th ed., revised June 2020 Biosafety in Microbiological and Biomedical Laboratories (BMBL) has become the overarching guidance document for the practice of biosafety in the U.S.— the mechanism for addressing the safe handling and containment of infectious microorganisms and hazardous biological materials. The principles of biosafety introduced in 1984 in the first edition of BMBL1 and carried through this edition remain steadfast. These principles are containment and risk assessment. The fundamentals of containment include the microbiological practices, safety equipment, and facility safeguards that protect laboratory workers, the environment, and the public from exposure to infectious microorganisms that are handled and stored in the laboratory. Risk assessment is the process that enables the appropriate selection of microbiological practices, safety equipment, and facility safeguards that can help prevent Laboratory-associated infections (LAI). The purpose of periodic updates of BMBL is to refine guidance based on new knowledge and experiences and to address contemporary issues that present new risks that confront laboratory workers and the public health. In this way, the guidance provided within the BMBL will continue to serve the microbio- logical and biomedical community as a relevant and valuable reference. CS308133-A CDC-BiosafetyMicrobiologicalBiomedicalLaboratories-2020-P.pdf
  • Content Notes:
    Section I. Introduction -- Section II. Biological Risk Assessment -- Section III. Principles of Biosafety -- Section IV. Laboratory Biosafety Level Criteria -- Section V. Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities -- Section VI. Principles of Laboratory Biosecurity -- Section VII. Occupational Health Support for Biomedical Research -- Section VIII. Agent Summary Statements -- Section VIII-A: Bacterial Agents -- Section VIII-B: Fungal Agents -- Section VIII-C: Parasitic Agents -- Section VIII-D: Rickettsial Agents -- Section VIII-E: Viral Agents -- Section VIII-F: Arboviruses and Related Zoonotic Viruses -- Section VIII-G: Toxin Agents -- Section VIII-H: Prion Diseases -- Appendix A. Primary Containment for Biohazards: Selection, Installation, and Use of Biological Safety Cabinets -- Appendix B. Decontamination and Disinfection of Laboratory Surfaces and Items -- Appendix C. Transportation of Infectious Substances -- Appendix D. Biosafety and Biocontainment for Pathogens Affecting Agricultural Animals and Animals that are Loose-Housed or in Open Penning -- Appendix E. Arthropod Containment Guidelines (ACG) -- Appendix F. Select Agents and Toxins -- Appendix G. Integrated Pest Management (IPM) -- Appendix H. Working with Human, Non-Human Primate (NHP), and Other Mammalian Cells and Tissues -- Appendix I. Guidelines for Work with Toxins of Biological Origin -- Appendix J. NIH Oversight of Research Involving Recombinant Biosafety Issues -- Appendix K. Inactivation and Verification -- Appendix L. Sustainability -- Appendix M. Large-Scale Biosafety -- Appendix N. Clinical Laboratories -- Appendix O. Acronyms -- Glossary -- Index -- Accessibility Descriptions of Figures -- Tables -- Figures.
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