Dioxin-like compound exposures and DNA methylation in the Anniston Community Health Survey Phase II

Details

• Alternative Title:
  Sci Total Environ
• Personal Author:
  Pittman, Gary S. ; Wang, Xuting ; Campbell, Michelle R. ; Coulter, Sherry J. ; Olson, James R. ; Pavuk, Marian ; Birnbaum, Linda S. ; Bell, Douglas A.
• Description:
  The Anniston Community Health Survey (ACHS-I) was initially conducted from 2005 to 2007 to assess polychlorinated biphenyl (PCB) exposures in Anniston, Alabama residents. In 2014, a follow-up study (ACHS-II) was conducted to measure the same PCBs as in ACHS-I and additional compounds e.g., polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like non-ortho (cPCBs) substituted PCBs. In this epigenome-wide association study (EWAS), we examined the associations between PCDD, PCDF, and PCB exposures and DNA methylation. Whole blood DNA methylation was measured using Illumina EPIC arrays (n=292). We modeled lipid-adjusted toxic equivalencies (TEQs) for: ΣDioxins (sum of 28 PCDDs, PCDFs, cPCBs, and mPCBs), PCDDs, PCDFs, cPCBs, and mPCBs using robust multivariable linear regression adjusting for age, race, sex, smoking, bisulfite conversion batch, and estimated percentages of six blood cell types. Among all exposures we identified 10 genome-wide (Bonferroni p≤6.74E-08) and 116 FDR (p≤5.00E-02) significant associations representing 10 and 113 unique CpGs, respectively. Of the 10 genome-wide associations, seven (70%) occurred in the PCDDs and four (40%) of these associations had an absolute differential methylation ≥1.00%, based on the methylation difference between the highest and lowest exposure quartiles. Most of the associations (six, 60%) represented hypomethylation changes. Of the 10 unique CpGs, eight (80%) were in genes shown to be associated with dioxins and/or PCBs based on data from the 2019 Comparative Toxicogenomics Database. In this study, we have identified a set of CpGs in blood DNA that may be particularly susceptible to dioxin, furan, and dioxin-like PCB exposures.
• Subjects:
  2,3,7,8-Tetrachlorodibenzodioxin (TCDD/dioxin) Alabama Anniston Community Health Survey (ACHS) Article Benzofurans Dibenzofurans, Polychlorinated Dioxins DNA Methylation Follow-Up Studies Mono-ortho PCBs Non-ortho PCBs Persistent Environmental Pollutant (POP) Polychlorinated Biphenyls Polychlorinated Biphenyls (PCBs) Polychlorinated Dibenzo-p-dioxins (PCDDs) Polychlorinated Dibenzofurans (PCDFs) Public Health Surveys And Questionnaires

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• Source:
  Sci Total Environ. 742:140424
• Pubmed ID:
  32629249
• Pubmed Central ID:
  PMC7574543
• Document Type:
  Journal Article
• Funding:
  U50 TS473215/TS/ATSDR CDC HHSUnited States/ ; Z01 ES100475/ImNIH/Intramural NIH HHSUnited States/
• Volume:
  742
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:9524e3080a5a5fa1eef099cdc6f29812743ff81d673c1980cb62e0bc641de849
• Download URL:
  https://stacks.cdc.gov/view/cdc/95827/cdc_95827_DS1.pdf
• File Type:
  [PDF - 2.71 MB ]