A SIMPLE MODIFICATION TO THE MOSQUITO HOMOGENIZATION PROTOCOL SAFELY INACTIVATES WEST NILE VIRUS AND ALLOWS VIRUS DETECTION BY THE RAPID ANALYTE MEASUREMENT PLATFORM (RAMP®) ASSAY

Details

• Alternative Title:
  J Am Mosq Control Assoc
• Personal Author:
  BURKHALTER, KRISTEN L. ; BIGGERSTAFF, BRAD J. ; HORIUCHI, KALANTHE ; SAVAGE, HARRY M.
• Description:
  We evaluated the ability of the Rapid Analyte Measurement Platform (RAMP(®)) mosquito-grinding buffer to inactivate West Nile virus (WNV) by subjecting WNV-positive samples ground in RAMP buffer to incubation intervals ranging from 5 min to 60 min. At each time point an aliquot was removed and serially diluted in bovine albumin (BA)-1 cell culture media to stop the inactivation process by RAMP buffer. Each BA-1 sample was tested for viable virus using Vero 6-well cell culture plaque assay and observed for plaques. We observed very limited inactivation of WNV (1-2 log10 plaque-forming units/ml) by RAMP buffer. Concerned for RAMP operators who may be using this assay in low-level biocontainment facilities, we developed an alternate sample homogenization protocol using Triton X-100 detergent that ensures complete WNV inactivation without compromising the performance of the RAMP assay.
• Subjects:
  Animals Article Culex Insect Vectors Mosquito Mosquito Control RAMP® Test Triton X-100 Virus Inactivation West Nile Virus
• Source:
  J Am Mosq Control Assoc. 32(2):77-82
• Pubmed ID:
  27280345
• Pubmed Central ID:
  PMC7111560
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHS/United States
• Volume:
  32
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:25ed47511551169c9b43e87941092698cb26cd26bacfd06ad38cf406a5e1b7bf
• Download URL:
  https://stacks.cdc.gov/view/cdc/86391/cdc_86391_DS1.pdf
• File Type:
  [PDF - 394.76 KB ]