Details:

Alternative Title:
Int J Antimicrob Agents
Personal Author:
Johnson, Steven R. ; Grad, Yonatan ; Abrams, A. Jeanine ; Pettus, Kevin ; Trees, David L.
Johnson, Steven R. ; Grad, Yonatan ; Abrams, A. Jeanine ; Pettus, Kevin ; Trees, David L. Less -
Description:
The whole-genome sequences of 24 isolates of Neisseria gonorrhoeae with elevated minimum inhibitory concentrations (MICs) to azithromycin (≥2.0 µg/mL) were analyzed against a modified sequence derived from the whole-genome sequence of N. gonorrhoeae FA1090 to determine, by signal ratio, the number of mutant copies of the 23S rRNA gene and the copy number effect on 50S ribosome-mediated azithromycin resistance. Isolates that were predicted to contain four mutated copies were accurately identified compared with the results of direct sequencing. Fewer than four mutated copies gave less accurate results but were consistent with elevated MICs.
Subject:
[+]
23S RRNA
Anti-Bacterial Agents
Article
Azithromycin
DNA, Bacterial
DNA, Ribosomal
Drug Resistance, Bacterial
Gene Dosage
Genome
Microbial Sensitivity Tests
Neisseria Gonorrhoeae
Point Mutation
Resistance
RNA, Ribosomal, 23S
Sequence Analysis, DNA

Source:
Int J Antimicrob Agents. 49(2):252-254
Pubmed ID:
28038960
Pubmed Central ID:
PMC6927039
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:b707328f7f0c15c37ac875d2e0be23c404d81dcaa65b10277043d96c0e6ee1a9
File Type:

Supporting Files

• 	nihms-1062778.nxml	xml
  	NIHMS1062778-supplement-Appendix_S1__Supplementary_Data.xlsx	xlsx

More +