Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Dengue Capsid

Details

• Alternative Title:
  Open Virol J
• Personal Author:
  Selvarajah, Suganya ; Chatterji, Udayan ; Kuhn, Richard ; Kinney, Richard ; Vasudevan, Subhash G ; Gallay, Philippe
• Description:
  The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.
• Subjects:
  Article Capsid Dengue ELISA IFSA.
• Source:
  Open Virol J. 2012; 6:29-37.
• Document Type:
  Journal Article
• Volume:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:826b8c7f1f322a9b14fa191cb45374a0551fc14555ecf9f5dbc2c4997ba5dbc7
• Download URL:
  https://stacks.cdc.gov/view/cdc/8215/cdc_8215_DS1.pdf
• File Type:
  [PDF - 2.36 MB ]