Details:

Alternative Title:
Open Virol J
Personal Author:
Selvarajah, Suganya ; Chatterji, Udayan ; Kuhn, Richard ; Kinney, Richard ; Vasudevan, Subhash G ; ... More +
Selvarajah, Suganya ; Chatterji, Udayan ; Kuhn, Richard ; Kinney, Richard ; Vasudevan, Subhash G ; Gallay, Philippe Less -
Description:
The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.
Subjects:
[+]
Article Capsid Dengue ELISA IFSA.
Source:
Open Virol J. 2012; 6:29-37.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:826b8c7f1f322a9b14fa191cb45374a0551fc14555ecf9f5dbc2c4997ba5dbc7
Download URL:
https://stacks.cdc.gov/view/cdc/8215/cdc_8215_DS1.pdf
File Type:

Supporting Files

• 	TOVJ-6-29_F4.gif	gif
  	TOVJ-6-29_F4.jpg	jpeg
  	TOVJ-6-29_F5.gif	gif
  	TOVJ-6-29_F5.jpg	jpeg
  	license.txt	txt
  	TOVJ-6-29.nxml	txt
  	TOVJ-6-29_F1.gif	gif
  	TOVJ-6-29_F1.jpg	jpeg
  	TOVJ-6-29_F2.gif	gif
  	TOVJ-6-29_F2.jpg	jpeg
  	TOVJ-6-29_F3.gif	gif
  	TOVJ-6-29_F3.jpg	jpeg

More +