Details:

Alternative Title:
PLoS One
Personal Author:
Lima-Junior, Josué C. ; Rodrigues-da-Silva, Rodrigo N. ; Banic, Dalma M. ; Jiang, Jianlin ; Singh, Balwan ; ... More +
Lima-Junior, Josué C. ; Rodrigues-da-Silva, Rodrigo N. ; Banic, Dalma M. ; Jiang, Jianlin ; Singh, Balwan ; Fabrício-Silva, Gustavo M. ; Porto, Luís C. S. ; Meyer, Esmeralda V. S. ; Moreno, Alberto ; Rodrigues, Maurício M. ; Barnwell, John W. ; Galinski, Mary R. ; de Oliveira-Ferreira, Joseli Less -
Description:
Background

The antibody response generated during malaria infections is of particular interest, since the production of specific IgG antibodies is required for acquisition of clinical immunity. However, variations in antibody responses could result from genetic polymorphism of the HLA class II genes. Given the increasing focus on the development of subunit vaccines, studies of the influence of class II alleles on the immune response in ethnically diverse populations is important, prior to the implementation of vaccine trials.

Methods and Findings

In this study, we evaluated the influence of HLA-DRB1* and -DQB1* allelic groups on the naturally acquired humoral response from Brazilian Amazon individuals (n = 276) against P. vivax Merozoite Surface Protein-1 (MSP-1), MSP-3α and MSP-9 recombinant proteins. Our results provide information concerning these three P. vivax antigens, relevant for their role as immunogenic surface proteins and vaccine candidates. Firstly, the studied population was heterogeneous presenting 13 HLA-DRB1* and 5 DQB1* allelic groups with a higher frequency of HLA-DRB1*04 and HLA-DQB1*03. The proteins studied were broadly immunogenic in a naturally exposed population with high frequency of IgG antibodies against PvMSP1-19 (86.7%), PvMSP-3 (77%) and PvMSP-9 (76%). Moreover, HLA-DRB1*04 and HLA-DQB1*03 alleles were associated with a higher frequency of IgG immune responses against five out of nine antigens tested, while HLA-DRB1*01 was associated with a high frequency of non-responders to repetitive regions of PvMSP-9, and the DRB1*16 allelic group with the low frequency of responders to PvMSP3 full length recombinant protein.

Conclusions

HLA-DRB1*04 alleles were associated with high frequency of antibody responses to five out of nine recombinant proteins tested in Rondonia State, Brazil. These features could increase the success rate of future clinical trials based on these vaccine candidates.


Subjects:
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Adult Aged Aged, 80 And Over Antigens, Protozoan Child Ethnicity Gene Frequency HLA-DQ Beta-Chains HLA-DRB1 Chains Humans Immunoglobulin G Interviews As Topic Malaria, Vivax Membrane Proteins Merozoite Surface Protein 1 Middle Aged Prevalence Protozoan Proteins Recombinant Proteins
Source:
PLoS One. 2012; 7(5).
Document Type:
Journal Article
Place as Subject:
Brazil
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:92ae1fe666c437826e54b4fe250ba9f2f4b5471bcf6aebcb894aee8462fda658
Download URL:
https://stacks.cdc.gov/view/cdc/8188/cdc_8188_DS1.pdf
File Type:

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