A Non-VH1-69 Heterosubtypic Neutralizing Human Monoclonal Antibody Protects Mice against H1N1 and H5N1 Viruses

Details

• Alternative Title:
  PLoS One
• Personal Author:
  De Marco, Donata ; Clementi, Nicola ; Mancini, Nicasio ; Solforosi, Laura ; Moreno, Guisella J. ; Sun, Xiangjie ; Tumpey, Terrence M. ; Gubareva, Larisa V. ; Mishin, Vasiliy ; Clementi, Massimo ; Burioni, Roberto
• Description:
  Influenza viruses are among the most important human pathogens and are responsible for annual epidemics and sporadic, potentially devastating pandemics. The humoral immune response plays an important role in the defense against these viruses, providing protection mainly by producing antibodies directed against the hemagglutinin (HA) glycoprotein. However, their high genetic variability allows the virus to evade the host immune response and the potential protection offered by seasonal vaccines. The emergence of resistance to antiviral drugs in recent years further limits the options available for the control of influenza. The development of alternative strategies for influenza prophylaxis and therapy is therefore urgently needed. In this study, we describe a human monoclonal antibody (PN-SIA49) that recognizes a highly conserved epitope located on the stem region of the HA and able to neutralize a broad spectrum of influenza viruses belonging to different subtypes (H1, H2 and H5). Furthermore, we describe its protective activity in mice after lethal challenge with H1N1 and H5N1 viruses suggesting a potential application in the treatment of influenza virus infections.
• Subjects:
  Amino Acid Sequence Animals Antibodies, Monoclonal Antibodies, Viral Biology Epitopes Female Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins Humans Influenza A Virus, H1N1 Subtype Influenza A Virus, H5N1 Subtype Influenza Vaccines Medicine Mice Mice, Inbred BALB C Molecular Sequence Data Mutagenesis Mutation Neutralization Tests Orthomyxoviridae Infections Protein Conformation Research Article Sequence Homology, Amino Acid

  More +
• Source:
  PLoS One. 2012; 7(4)
• Pubmed ID:
  22496802
• Pubmed Central ID:
  PMC3319592
• Document Type:
  Journal Article
• Volume:
  7
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:581640d185f7fddf14ec934996985ea76cf7da454c8e41064d471d2da8901084
• Download URL:
  https://stacks.cdc.gov/view/cdc/8176/cdc_8176_DS1.pdf
• File Type:
  [PDF - 1.22 MB ]