Impaired Clearance and Enhanced Pulmonary Inflammatory/Fibrotic Response to Carbon Nanotubes in Myeloperoxidase-Deficient Mice

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Shvedova, Anna A. ; Kapralov, Alexandr A. ; Feng, Wei Hong ; Kisin, Elena R. ; Murray, Ashley R. ; Mercer, Robert R. ; St. Croix, Claudette M. ; Lang, Megan A. ; Watkins, Simon C. ; Konduru, Nagarjun V. ; Allen, Brett L. ; Conroy, Jennifer ; Kotchey, Gregg P. ; Mohamed, Bashir M. ; Meade, Aidan D. ; Volkov, Yuri ; Star, Alexander ; Fadeel, Bengt ; Kagan, Valerian E.
• Description:
  Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations.
• Subjects:
  Animals Bronchoalveolar Lavage Fluid Chemokine CCL2 Female Fibrosis Interleukin-6 Lung Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Knockout Microscopy, Electron, Transmission Microscopy, Fluorescence Nanotubes, Carbon Neutrophils Oxidation-Reduction Peroxidase Pneumonia Spectrum Analysis, Raman Tumor Necrosis Factor-alpha

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• Source:
  PLoS One. 2012; 7(3).
• Pubmed ID:
  22479306
• Pubmed Central ID:
  PMC3316527
• Document Type:
  Journal Article
• Funding:
  HL094488/HL/NHLBI NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; LM007994-05/LM/NLM NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; R01 ES019304/ES/NIEHS NIH HHS/United States ; R01ES019304/ES/NIEHS NIH HHS/United States ; U19AI068021/AI/NIAID NIH HHS/United States ; U54 GM103529/GM/NIGMS NIH HHS/United States
• Volume:
  7
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e3a021619fa4d795b67370b632683e7705e20a6e331540665cb6d0c088fe019a
• Download URL:
  https://stacks.cdc.gov/view/cdc/8143/cdc_8143_DS1.pdf
• File Type:
  [PDF - 528.83 KB ]