Carbapenem Resistance in Klebsiella pneumoniae Not Detected by Automated Susceptibility Testing

Tenover, Fred C.; Kalsi, Rajinder K.; Williams, Portia P.; Carey, Roberta B.; Stocker, Sheila; Lonsway, David; Rasheed, J. Kamile; Biddle, James W.; McGowan, John E.; Hanna, Bruce

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Carbapenem Resistance in Klebsiella pneumoniae Not Detected by Automated Susceptibility Testing

Supporting Files Public Domain

Aug 2006
By Tenover, Fred C. ; Kalsi, Rajinder K. ; Williams, Portia P. ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Tenover, Fred C. ; Kalsi, Rajinder K. ; Williams, Portia P. ; Carey, Roberta B. ; Stocker, Sheila ; Lonsway, David ; Rasheed, J. Kamile ; Biddle, James W. ; McGowan, John E. ; Hanna, Bruce
Description:

Detecting beta-lactamase-mediated carbapenem resistance among Klebsiella pneumoniae isolates and other Enterobacteriaceae is an emerging problem. In this study, 15 blaKPC-positive Klebsiella pneumoniae that showed discrepant results for imipenem and meropenem from 4 New York City hospitals were characterized by isoelectric focusing; broth microdilution (BMD); disk diffusion (DD); and MicroScan, Phoenix, Sensititre, VITEK, and VITEK 2 automated systems. All 15 isolates were either intermediate or resistant to imipenem and meropenem by BMD; 1 was susceptible to imipenem by DD. MicroScan and Phoenix reported 1 (6.7%) and 2 (13.3%) isolates, respectively, as imipenem susceptible. VITEK and VITEK 2 reported 10 (67%) and 5 (33%) isolates, respectively, as imipenem susceptible. By Sensititre, 13 (87%) isolates were susceptible to imipenem, and 12 (80%) were susceptible to meropenem. The VITEK 2 Advanced Expert System changed 2 imipenem MIC results from >16 ?g/mL to <2 ?g/mL but kept the interpretation as resistant. The recognition of carbapenem-resistant K. pneumoniae continues to challenge automated susceptibility systems.
Subjects:

Anti-Bacterial Agents Automation Carbapenems Drug Resistance, Bacterial Humans Imipenem Klebsiella Pneumoniae Microbial Sensitivity Tests Reference Standards Research Thienamycins
Source:

Emerg Infect Dis. 12(8):1209-1213.
Document Type:

Journal Article
Volume:

12
Issue:

8
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:f52c683d07239fb42eab18853bff0d9e69941e5c7d4fb95d37d474623d3a0091
Download URL:

https://stacks.cdc.gov/view/cdc/7927/cdc_7927_DS1.pdf
File Type:

[PDF - 105.46 KB ]

File Language:

English

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