Label-Free and Continuous-Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids

Details

• Alternative Title:
  Adv Funct Mater
• Personal Author:
  Zhao, Wujun ; Zhu, Taotao ; Cheng, Rui ; Liu, Yufei ; He, Jian ; Qiu, Hong ; Wang, Lianchun ; Nagy, Tamas ; Querec, Troy D. ; Unger, Elizabeth R. ; Mao, Leidong
• Description:
  In this study, a label-free, low-cost, and fast ferrohydrodynamic cell separation scheme is demonstrated using HeLa cells (an epithelial cell line) and red blood cells. The separation is based on cell size difference, and conducted in a custom-made biocompatible ferrofluid that retains the viability of cells during and after the assay for downstream analysis. The scheme offers moderate-throughput (≈10| cells h| for a single channel device) and extremely high recovery rate (>99%) without the use of any label. It is envisioned that this separation scheme will have clinical applications in settings where rapid cell enrichment and removal of contaminating blood will improve efficiency of screening and diagnosis such as cervical cancer screening based on mixed populations in exfoliated samples.
• Source:
  Adv Funct Mater. 26(22):3990-3998
• Pubmed ID:
  27478429
• Pubmed Central ID:
  PMC4963013
• Document Type:
  Journal Article
• Funding:
  R21 HL131553/HL/NHLBI NIH HHSUnited States/ ; R01 HL093339/HL/NHLBI NIH HHSUnited States/ ; R21 GM104528/GM/NIGMS NIH HHSUnited States/ ; P41 GM103390/GM/NIGMS NIH HHSUnited States/ ; CC999999/Intramural CDC HHSUnited States/ ; P41 RR005351/RR/NCRR NIH HHSUnited States/
• Volume:
  26
• Issue:
  22
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:488f7c63955de638700f838c425b6969468be60fda12441f8272a5e0348708b9
• Download URL:
  https://stacks.cdc.gov/view/cdc/78641/cdc_78641_DS1.pdf
• File Type:
  [PDF - 785.66 KB ]