Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity

Wormser, Gary P.; Molins, Claudia R.; Levin, Andrew; Lipsett, Susan C.; Nigrovic, Lise E.; Schriefer, Martin E.; Branda, John A.

doi:10.1016/j.diagmicrobio.2018.02.006

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Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity

February 16 2018
By Wormser, Gary P. ; Molins, Claudia R. ; Levin, Andrew ; ...
http://dx.doi.org/10.1016/j.diagmicrobio.2018.02.006
Source: Diagn Microbiol Infect Dis. 91(3):217-219

[PDF-49.97 KB]

English

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Details:

Alternative Title:

Diagn Microbiol Infect Dis
Personal Author:

Wormser, Gary P. ; Molins, Claudia R. ; Levin, Andrew ; Lipsett, Susan C. ; Nigrovic, Lise E. ; ... More +

Wormser, Gary P. ; Molins, Claudia R. ; Levin, Andrew ; Lipsett, Susan C. ; Nigrovic, Lise E. ; Schriefer, Martin E. ; Branda, John A. Less -
Description:

To diagnose Lyme disease, a two-tier testing algorithm is used in which supplemental IgM and IgG immunoblots to detect antibody to Borrelia burgdorferi are reflexively performed if a first-tier assay, such as a whole-cell sonicate-based enzyme immunoassay (WCS EIA), is reactive. Recent data suggest that equal specificity is found by substituting the C6 peptide EIA for immunoblots. In this study using 3956 control sera, we demonstrated that although this two-tier testing algorithm does significantly improve diagnostic specificity compared with each of the EIAs individually, the WCS EIA and the C6 peptide EIA are not independent tests. Therefore, when the C6 peptide EIA is used as the second-tier test, it should be regarded as a supplemental rather than a confirmatory test.
Subjects:

[+]

Algorithms Antibodies, Bacterial Article Borrelia Burgdorferi C6 Lyme Test Humans Immunoenzyme Techniques Immunoglobulin G Immunoglobulin M Lyme Disease Sensitivity And Specificity Test Independence Two-tier Serology
Source:

Diagn Microbiol Infect Dis. 91(3):217-219
Pubmed ID:

29550060
Pubmed Central ID:

PMC6519922
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHS/United States
Volume:

91
Issue:

3
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:e70763cd6cd9917661630f13cbfa49d3c1711f75f53215281daec7a077c46f2a
Download URL:

https://stacks.cdc.gov/view/cdc/78530/cdc_78530_DS1.pdf
File Type:

nihms-1024769.nxml

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