i
Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines
-
July 12 2018
Source: Virology. 522:65-72 -
Alternative Title:Virology
-
Publisher's site:
-
Personal Author:
-
Description:Cold adapted influenza virus A/Leningrad/134/17/57 (H2N2) is a reliable master donor virus (Len/17-MDV) for preparing live attenuated influenza vaccines (LAIV). LAIVs are 6:2 reasortants that contain 6 segments of Len/17-MDV and the hemagglutinin (HA) and neuraminidase (NA) of contemporary circulating influenza A viruses. The problem with the classical reassortment procedure used to generate LAIVs is that there is limited selection pressure against NA of the Len/17-MDV resulting in 7:1 reassortants with desired HA only, which are not suitable LAIVs. The monoclonal antibodies (mAb) directed against the N2 of Len/17-MDV were generated. 10C4-8E7 mAb inhibits cell-to-cell spread of viruses containing the Len/17-MDV N2, but not viruses with the related N2 from contemporary H3N2 viruses. 10C4-8E7 antibody specifically inhibited the Len/17-MDV replication in vitro and in ovo but didn't inhibit replication of H3N2 or H1N1pdm09 reassortants. Our data demonstrate that addition of 10C4-8E7 in the classical reassortment improves efficiency of LAIV production.
-
Subject:
-
Source:
-
Pubmed ID:30014859
-
Pubmed Central ID:PMC6498426
-
Document Type:
-
Collection(s):
-
Main Document Checksum:
-
File Type:
-
gif 
jpeg 
gif 
jpeg 
xml 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg
[PDF-964.72 KB]
Details:
Supporting Files
More +
Email
CDC-INFO
