Bacteriophage K Antimicrobial-Lock Technique for Treatment of Staphylococcus aureus Central Venous Catheter–Related Infection: A Leporine Model Efficacy Analysis

Details

• Alternative Title:
  J Vasc Interv Radiol
• Personal Author:
  Lungren, Matthew P. ; Donlan, Rodney M. ; Kankotia, Ravi ; Paxton, Ben E. ; Falk, Irene ; Christensen, Diana ; Kim, Charles Y.
• Description:
  Purpose:
  
  To determine whether a bacteriophage antimicrobial-lock technique can reduce bacterial colonization and biofilm formation on indwelling central venous catheters in a rabbit model.
  
  Materials and Methods:
  
  Cuffed central venous catheters were inserted into the jugular vein of female New Zealand White rabbits under image guidance. Catheters were inoculated for 24 hours with broth culture of methicillin-sensitive Staphylococcus aureus. The inoculum was aspirated, and rabbits were randomly assigned to two equal groups for 24 hours: (i) untreated controls (heparinized saline lock), (ii) bacteriophage antimicrobial-lock (staphylococcal bacteriophage K, propagated titer > 108/mL). Blood cultures were obtained via peripheral veins, and the catheters were removed for quantitative culture and scanning electron microscopy.
  
  Results:
  
  Mean colony-forming units (CFU) per cm2 of the distal catheter segment, as a measure of biofilm, were significantly decreased in experimental animals compared with controls (control, 1.2 × 105 CFU/cm2; experimental, 7.6 × 103; P = .016). Scanning electron microscopy demonstrated that biofilms were present on the surface of five of five control catheters but only one of five treated catheters (P = .048). Blood culture results were not significantly different between the groups.
  
  Conclusions:
  
  In a rabbit model, treatment of infected central venous catheters with a bacteriophage antimicrobial-lock technique significantly reduced bacterial colonization and biofilm presence. Our data represent a preliminary step toward use of bacteriophage therapy for prevention and treatment of central venous catheter–associated infection.
  
  
• Subjects:
  Animals Article Bacteriophages Biofilms Catheter-Related Infections Catheterization, Central Venous Central Venous Catheters Disease Models, Animal Equipment Design Female Jugular Veins Rabbits Staphylococcal Infections Staphylococcus Aureus

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• Source:
  J Vasc Interv Radiol. 25(10):1627-1632
• Pubmed ID:
  25088065
• Pubmed Central ID:
  PMC6467293
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  25
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f5fffea0c672bc07ac87f288474692ae9e3020e7025598aa9776e0d094fafe76
• Download URL:
  https://stacks.cdc.gov/view/cdc/77596/cdc_77596_DS1.pdf
• File Type:
  [PDF - 529.96 KB ]