Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons

Details

• Alternative Title:
  Euro Surveill
• Personal Author:
  Gubareva, Larisa V ; Mishin, Vasiliy P ; Patel, Mira C ; Chesnokov, Anton ; Nguyen, Ha T ; De La Cruz, Juan ; Spencer, Sarah ; Campbell, Angela P ; Sinner, Mallory ; Reid, Heather ; Garten, Rebecca ; Katz, Jackie M ; Fry, Alicia M ; Barnes, John ; Wentworth, David E
• Description:
  The anti-influenza therapeutic baloxavir targets cap-dependent endonuclease activity of polymerase acidic (PA) protein. We monitored baloxavir susceptibility in the United States with next generation sequencing analysis supplemented by phenotypic one-cycle infection assay. Analysis of PA sequences of 6,891 influenza A and B viruses collected during 2016/17 and 2017/18 seasons showed amino acid substitutions: I38L (two A(H1N1)pdm09 viruses), E23G (two A(H1N1)pdm09 viruses) and I38M (one A(H3N2) virus); conferring 4-10-fold reduced susceptibility to baloxavir.
• Subjects:
  Cap-dependent Endonuclease Inhibitor Drug Resistance Influenza Influenza Surveillance Next Generation Sequencing Phenotypic Testing Polymerase Acidic Protein Rapid Communication
• Source:
  Euro Surveill. 2019; 24(3)
• Pubmed ID:
  30670144
• Pubmed Central ID:
  PMC6344838
• Document Type:
  Journal Article
• Volume:
  24
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:fa2b24ed2a253dec7441247b0d063a89915edfa377dd722ceeaa7dd02c98207f
• Download URL:
  https://stacks.cdc.gov/view/cdc/76161/cdc_76161_DS1.pdf
• File Type:
  [PDF - 142.93 KB ]