Details:

Alternative Title:
Virulence
Publisher's site:
http://dx.doi.org/10.1080/21505594.2018.1499379
Personal Author:
Paes, Jéssica Andrade ; Machado, Lais Del Prá Netto ; dos Anjos Leal, Fernanda Munhoz ; De Moraes, Sofia Nóbrega ; Moura, Hercules ; ... More +
Paes, Jéssica Andrade ; Machado, Lais Del Prá Netto ; dos Anjos Leal, Fernanda Munhoz ; De Moraes, Sofia Nóbrega ; Moura, Hercules ; Barr, John R. ; Ferreira, Henrique Bunselmeyer Less -
Description:
Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia (PEP), M. flocculare is a commensal species. To identify potential PEP determinants and provide novel insights on mycoplasma-host interactions, the whole cell proteomes of two M. hyopneumoniae strains, one pathogenic (7448) and other non-pathogenic (J), and M. flocculare were compared. A cell fractioning approach combined with mass spectrometry (LC-MS/MS) proteomics was used to analyze cytoplasmic and surface-enriched protein fractions. Average detection of ~ 50% of the predicted proteomes of M. hyopneumoniae 7448 and J, and M. flocculare was achieved. Many of the identified proteins were differentially represented in M. hyopneumoniae 7448 in comparison to M. hyopneumoniae J and M. flocculare, including potential PEP determinants, such as adhesins, proteases, and redox-balancing proteins, among others. The LC-MS/MS data also provided experimental validation for several genes previously regarded as hypothetical for all analyzed mycoplasmas, including some coding for proteins bearing virulence-related functional domains. The comprehensive proteome profiling of two M. hyopneumoniae strains and M. flocculare provided tens of novel candidates to PEP determinants or virulence factors, beyond those classically described.
Subject:
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Bacterium-host Interactions
Comparative Proteomics
Mycoplasma
Research Paper
Subcellular Fractions
Swine Respiratory Pathogens
Virulence Factors

Source:
Virulence. 2018; 9(1):1230-1246.
Pubmed ID:
30027802
Pubmed Central ID:
PMC6104684
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:6167db2a7518f3ee85e4e8e326157fab8aea70082d1bd1c92b599b00b9b714c7
File Type:

Supporting Files

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