Identification of human vaccinees that possess antibodies targeting the egg-adapted hemagglutinin receptor binding site of an H1N1 influenza vaccine strain

Garretson, Tyler A.; Petrie, Joshua G.; Martin, Emily T.; Monto, Arnold S.; Hensley, Scott E.

doi:10.1016/j.vaccine.2018.05.086

i

Identification of human vaccinees that possess antibodies targeting the egg-adapted hemagglutinin receptor binding site of an H1N1 influenza vaccine strain

Supporting Files

May 31 2018
By Garretson, Tyler A. ; Petrie, Joshua G. ; Martin, Emily T. ; ...

File Language:

English

Details

Alternative Title:

Vaccine
Personal Author:

Garretson, Tyler A. ; Petrie, Joshua G. ; Martin, Emily T. ; Monto, Arnold S. ; Hensley, Scott E.
Description:

Human influenza viruses passaged in eggs often acquire mutations in the hemagglutinin (HA) receptor binding site (RBS). To determine if egg-adapted H1N1 vaccines commonly elicit antibodies targeting the egg-adapted RBS of HA, we completed hemagglutinin-inhibition assays with A/California/7/2009 HA and egg-adapted A/California/7/2009-X-179A HA using sera collected from 159 humans vaccinated with seasonal influenza vaccines during the 2015-16 season. We found that ∼5% of participants had ≥4-fold higher antibody titers to the egg-adapted viral strain compared to wild type viral strain. We used reverse-genetics to demonstrate that a single egg-adapted HA RBS mutation (Q226R) was responsible for this phenotype.
Subjects:

Adolescent Adult Aged Antibodies Antibodies, Viral Article Cohort Studies Female Hemagglutination Inhibition Tests Hemagglutinin Humans Influenza Influenza A Virus, H1N1 Subtype Influenza Vaccines Male Middle Aged Vaccines Young Adult
Source:

Vaccine. 36(28):4095-4101
Pubmed ID:

29861178
Pubmed Central ID:

PMC5995672
Document Type:

Journal Article
Funding:

R01 AI108686/AI/NIAID NIH HHS/United States ; R56 AI097150/AI/NIAID NIH HHS/United States ; R01 AI097150/AI/NIAID NIH HHS/United States ; R01 AI113047/AI/NIAID NIH HHS/United States ; HHSN272201400005C/AI/NIAID NIH HHS/United States ; U01 IP000474/IP/NCIRD CDC HHS/United States
Volume:

36
Issue:

28
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:4b20855a966446fec665840cf1766c75cad45947a65b81bf287b1a41f109f2f3
Download URL:

https://stacks.cdc.gov/view/cdc/55849/cdc_55849_DS1.pdf
File Type:

[PDF - 695.94 KB ]

File Language:

English

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