Details:

Alternative Title:
Am J Trop Med Hyg
Publisher's site:
http://dx.doi.org/10.4269/ajtmh.17-0421
Personal Author:
Hercik, Christine ; Cosmas, Leonard ; Mogeni, Ondari D. ; Wamola, Newton ; Kohi, Wanze ; ... More +
Hercik, Christine ; Cosmas, Leonard ; Mogeni, Ondari D. ; Wamola, Newton ; Kohi, Wanze ; Houpt, Eric ; Liu, Jie ; Ochieng, Caroline ; Onyango, Clayton ; Fields, Barry ; Mfinanga, Sayoki ; Montgomery, Joel M. Less -
Description:
The use of fever syndromic surveillance in sub-Saharan Africa is an effective approach to determine the prevalence of both malarial and nonmalarial infectious agents. We collected both blood and naso/oro-pharyngeal (NP/OP) swabs from consecutive consenting patients ≥ 1 year of age, with an axillary temperature ≥ 37.5°C, and symptom onset of ≤ 5 days. Specimens were analyzed using both acute febrile illness (AFI) and respiratory TaqMan array cards (Resp TAC) for multiagent detection of 56 different bloodstream and respiratory agents. In addition, we collected epidemiologic data to further characterize our patient population. We enrolled 205 febrile patients, including 70 children (1 < 15 years of age; 34%) and 135 adults (≥ 15 years of age; 66%). AFI TAC and Resp TAC were performed on 191 whole blood specimens and 115 NP/OP specimens, respectively. We detected nucleic acid for | (57%), | (2%), and dengue virus (1%) among blood specimens. In addition, we detected 17 different respiratory agents, most notably, | (64%), | (56%), | (39%), and respiratory syncytial virus (11%) among NP/OP specimens. Overall median cycle threshold was measured at 26.5. This study provides a proof-of-concept for the use of a multiagent diagnostic approach for exploratory research on febrile illness and underscores the utility of quantitative molecular diagnostics in complex epidemiologic settings of sub-Saharan Africa.
Subjects:
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Adolescent Adult Child Child, Preschool Cross-Sectional Studies Female Fever Humans Male Middle Aged Pilot Projects Rural Population Syndrome
Source:
Am J Trop Med Hyg. 2018; 98(2):625-632.
Pubmed ID:
29280432
Pubmed Central ID:
PMC5929188
Document Type:
Journal Article
Place as Subject:
Tanzania
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:d3b2d768c3677df171f5196dc5aa793dd064381d6313e49cc83e2be91e1a7ab0
Download URL:
https://stacks.cdc.gov/view/cdc/53880/cdc_53880_DS1.pdf
File Type:

Supporting Files

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