Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient

Dhenni, Rama; Karyanti, Mulya Rahma; Putri, Nina Dwi; Yohan, Benediktus; Yudhaputri, Frilasita A.; Ma'roef, Chairin Nisa; Fadhilah, Araniy; Perkasa, Aditya; Restuadi, Restuadi; Trimarsanto, Hidayat; Mangunatmadja, Irawan; Ledermann, Jeremy P.; Rosenberg, Ronald; Powers, Ann M.; Myint, Khin Saw Aye; Sasmono, R. Tedjo

doi:10.1371/journal.pntd.0006198

i

Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient

Supporting Files

Jan 12 2018
By Dhenni, Rama ; Karyanti, Mulya Rahma ; Putri, Nina Dwi ; ...

File Language:

English

Details

Alternative Title:

PLoS Negl Trop Dis
Personal Author:

Dhenni, Rama ; Karyanti, Mulya Rahma ; Putri, Nina Dwi ; Yohan, Benediktus ; Yudhaputri, Frilasita A. ; Ma'roef, Chairin Nisa ; Fadhilah, Araniy ; Perkasa, Aditya ; Restuadi, Restuadi ; Trimarsanto, Hidayat ; Mangunatmadja, Irawan ; Ledermann, Jeremy P. ; Rosenberg, Ronald ; Powers, Ann M. ; Myint, Khin Saw Aye ; Sasmono, R. Tedjo
Description:

Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fluid of an encephalitis paediatric patient in Jakarta, Indonesia. Next-generation sequencing was employed to deduce the complete genome of the neurotropic DENV-3 isolate. Based on complete genome analysis, two unique and nine uncommon amino acid changes in the protein coding region were observed in the virus. A phylogenetic tree and molecular clock analysis revealed that the neurotropic virus was a member of Sumatran-Javan clade of DENV-3 genotype I and shared a common ancestor with other isolates from Jakarta around 1998. This is the first report of neurotropic DENV-3 complete genome analysis, providing detailed information on the genetic characteristics of this virus.
Subjects:

Amino Acid Substitution Cerebrospinal Fluid Computational Biology Dengue Dengue Virus Encephalitis, Viral Evolution, Molecular Genome, Viral High-Throughput Nucleotide Sequencing Humans Indonesia Mutation, Missense Phylogeny Sequence Analysis, DNA Serogroup
Source:

PLoS Negl Trop Dis. 12(1).
Pubmed ID:

29329287
Pubmed Central ID:

PMC5809095
Document Type:

Journal Article
Funding:

U18 CK000443/CK/NCEZID CDC HHS/United States
Volume:

12
Issue:

1
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:9b80e1168afa5dc90b1c57c51ea4e61dbebeb5a49a71714c3c935d82ee296f56
Download URL:

https://stacks.cdc.gov/view/cdc/52917/cdc_52917_DS1.pdf
File Type:

[PDF - 4.89 MB ]

File Language:

English

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