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Development of a consensus method for culture of Clostridium difficile from meat and its use in a survey of U.S. retail meats
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Aug 21 2012
Source: Food Microbiol. 32(2):448-451. -
Alternative Title:Food Microbiol
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Description:Three previously described methods for culture of Clostridium difficile from meats were evaluated by microbiologists with experience in C. difficile culture and identification. A consensus protocol using BHI broth enrichment followed by ethanol shock and plating to selective and non-selective media was selected for use, and all participating laboratories received hands-on training in the use of this method prior to study initiation. Retail meat products (N = 1755) were cultured for C. difficile over 12 months during 2010-2011 at 9 U.S. FoodNet sites. No C. difficile was recovered, although other clostridia were isolated.
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Pubmed ID:22986214
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Pubmed Central ID:PMC5755364
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