Details:

Alternative Title:
Virology
Personal Author:
Wilson, Jason R. ; Guo, Zhu ; Tzeng, Wen-Pin ; Garten, Rebecca J. ; Xiyan, Xu ; ... More +
Wilson, Jason R. ; Guo, Zhu ; Tzeng, Wen-Pin ; Garten, Rebecca J. ; Xiyan, Xu ; Blanchard, Elisabeth G. ; Blanchfield, Kristy ; Stevens, James ; Katz, Jacqueline M. ; York, Ian A. Less -
Description:
Here we define the epitopes on HA that are targeted by a group of 9 recombinant monoclonal antibodies (rmAbs) isolated from memory B cells of mice, immunized by infection with A(H1N1)pdm09 virus followed by a seasonal TIV boost. These rmAbs were all reactive against the HA1 region of HA, but display 7 distinct binding footprints, targeting each of the 4 known antigenic sites. Although the rmAbs were not broadly cross-reactive, a group showed subtype-specific cross-reactivity with the HA of A/South Carolina/1/18. Screening these rmAbs with a panel of human A(H1N1)pdm09 virus isolates indicated that naturally-occurring changes in HA could reduce rmAb binding, HI activity, and/or virus neutralization activity by rmAb, without showing changes in recognition by polyclonal antiserum. In some instances, virus neutralization was lost while both ELISA binding and HI activity were retained, demonstrating a discordance between the two serological assays traditionally used to detect antigenic drift.
Subject:
[+]
A/H1N1/pdm09
Animals
Antibodies, Monoclonal
Antibodies, Viral
Antigenic Drift
Antigenic Site
Antigenic Variation
Antigens, Viral
Article
Binding Sites
Cross Reactions
Epitope Mapping
Epitopes
Hemagglutinin Glycoproteins, Influenza Virus
Humans
Immunologic Memory
Influenza
Influenza, Human
Influenza A Virus, H1N1 Subtype
Mice
Models, Molecular
Monoclonal Antibody
Orthomyxoviridae Infections
Protein Binding
Protein Conformation
Recombinant Proteins
Virus Neutralization
Epitope Mapping

Source:
Virology. 485:252-262
Pubmed ID:
26318247
Pubmed Central ID:
PMC5737639
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:79a1c0089f0d83dc80a96dec81ee4c448fb168de928c3acd3d874dfb2d4e7623
File Type:

Supporting Files

• 	nihms924344f3.gif	gif
  	nihms924344f3.jpg	jpeg
  	nihms924344f4.gif	gif
  	nihms924344f4.jpg	jpeg
  	NIHMS924344-supplement-1.docx	docx
  	nihms924344.nxml	xml
  	nihms924344f1.gif	gif
  	nihms924344f1.jpg	jpeg
  	nihms924344f2a.gif	gif
  	nihms924344f2a.jpg	jpeg
  	nihms924344f2b.gif	gif
  	nihms924344f2b.jpg	jpeg

More +