Details:

Alternative Title:
Virology
Publisher's site:
http://dx.doi.org/10.1016/j.virol.2017.08.016
Personal Author:
Wilson, Jason R. ; Belser, Jessica A. ; DaSilva, Juliana ; Guo, Zhu ; Sun, Xiangjie ; ... More +
Wilson, Jason R. ; Belser, Jessica A. ; DaSilva, Juliana ; Guo, Zhu ; Sun, Xiangjie ; Gansebom, Shane ; Bai, Yaohui ; Stark, Thomas J. ; Chang, Jessie ; Carney, Paul ; Levine, Min Z. ; Barnes, John ; Stevens, James ; Maines, Taronna R. ; Tumpey, Terrence M. ; York, Ian A. ; Less -
Description:
The emergence of A(H7N9) virus strains with resistance to neuraminidase (NA) inhibitors highlights a critical need to discover new countermeasures for treatment of A(H7N9) virus-infected patients. We previously described an anti-NA mAb (3c10-3) that has prophylactic and therapeutic efficacy in mice lethally challenged with A(H7N9) virus when delivered intraperitoneally (i.p.). Here we show that intrananasal (i.n.) administration of 3c10-3 protects 100% of mice from mortality when treated 24h post-challenge and further characterize the protective efficacy of 3c10-3 using a nonlethal A(H7N9) challenge model. Administration of 3c10-3 i.p. 24h prior to challenge resulted in a significant decrease in viral lung titers and deep sequencing analysis indicated that treatment did not consistently select for viral variants in NA. Furthermore, prophylactic administration of 3c10-3 did not inhibit the development of protective immunity to subsequent homologous virus re-challenge. Taken together, 3c10-3 highlights the potential use of anti-NA mAb to mitigate influenza virus infection.
Subject:
[+]
A(H7N9)
Administration, Intranasal
Animals
Antibodies, Monoclonal
Antibodies, Viral
Antiviral
Article
Avian Influenza
Disease Models, Animal
Immunologic Factors
Immunotherapeutic
Influenza
Influenza A Virus, H7N9 Subtype
Lung
Mice
Monoclonal Antibody
Neuraminidase
Orthomyxoviridae Infections
Passive Transfer
Survival Analysis
Treatment Outcome

Source:
Virology. 511:214-221. ;
Pubmed ID:
28888111
Pubmed Central ID:
PMC5727915
Document Type:
Journal Article ;
Funding:
CC999999/ImCDC/Intramural CDC HHS/United States ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c4eaef9aa390cfd1b3c2b66abc62f4c5310547748d4a859976b6c75bf0a82289
File Type:

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