Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic

Details

• Alternative Title:
  J Gen Virol
• Personal Author:
  Esona, Mathew D. ; Roy, Sunando ; Rungsrisuriyachai, Kunchala ; Sanchez, Jacqueline ; Vasquez, Lina ; Gomez, Virgen ; Rios, Lourdes Aviles ; Bowen, Michael D. ; Vazquez, Marietta
• Description:
  We report the genome of a novel human triple-recombinant G4P6-8_R mono-reassortant strain identified in a stool sample from the Dominican Republic during routine facility-based rotavirus strain surveillance. The strain was designated as RVA/Human-wt/DOM/2013840364/2013/G4P6-8_R, with a genomic constellation of G4-P6-8_R-I1-R1-C1-M1-(A1-A8_R)-N1-(T1-T7_R)-E1-H1. Recombinant gene segments NSP1 and NSP3 were generated as a result of recombination between genogroup 1 rotavirus A1 human strain and a genotype A8 porcine strain and between genogroup 1 rotavirus T1 human strain and a genotype T7 bovine strain, respectively. Analyses of the RNA secondary structures of gene segment VP4, NSP1 and NSP3 showed that all the recombinant regions appear to start in a loop (single-stranded) region and terminate in a stem (double-stranded) structure. Also, the VP7 gene occupied lineage VII within the G4 genotypes consisting of mostly porcine or porcine-like G4 strains, suggesting the occurrence of reassortment. The remaining gene segments clustered phylogenetically with genogroup 1 strains. This exchange of whole or partial genetic materials between rotaviruses by recombination and reassortment contributes directly to their diversification, adaptation and evolution.
• Subjects:
  • Article
  • rotavirus
  • triple recombinant
  • reassortment
  • Adaptation, Physiological
  • Animals
  • Cattle
  • Dominican Republic
  • Epidemiological Monitoring
  • Evolution, Molecular
  More +
• Source:
  J Gen Virol. 98(2):134-142
• Pubmed ID:
  27983480
• Pubmed Central ID:
  PMC5720362
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  98
• Issue:
  2
• Download URL:
  https://stacks.cdc.gov/view/cdc/50499/cdc_50499_DS1.pdf
• File Type:
  [PDF - 1.97 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a61ab55147537d8d187c1b3f88b950809d65555d54662bebe830fc1269eb27f6