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Albumin adsorption onto surfaces of urine collection and analysis containers☆
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Feb 07 2014
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Source: Clin Chim Acta. 431:40-45.
Details:
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Alternative Title:Clin Chim Acta
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Personal Author:
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Description:Background
Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations.
Methods
We added 125I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting.
Results
Adsorption of urine albumin (UA) at 5–6 mg/l was <0.9%; and at 90 mg/l was <0.4%. Adsorption was generally less at pH 8 than pH 5 but only 3 cases had p <0.05. Adsorption from 11 unaltered urine samples with albumin 5–333 mg/l was <0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100 ml and 2 l collection containers, and to instrument sample cups and showed <1% change in concentration at 5 mg/l and <0.5% change at 20 mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2–28%) was larger than that from urine.
Conclusions
Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.
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Source:
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Pubmed ID:24513540
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Pubmed Central ID:PMC5708857
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Volume:431
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