Albumin adsorption onto surfaces of urine collection and analysis containers☆

Details

• Alternative Title:
  Clin Chim Acta
• Personal Author:
  Robinson, Mary K. ; Caudill, Samuel P. ; Koch, David D. ; Ritchie, James ; Hortin, Glen ; Eckfeldt, John H. ; Sandberg, Sverre ; Williams, Desmond ; Myers, Gary ; Miller, W. Greg
• Description:
  Background
  
  Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations.
  
  Methods
  
  We added 125I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting.
  
  Results
  
  Adsorption of urine albumin (UA) at 5–6 mg/l was <0.9%; and at 90 mg/l was <0.4%. Adsorption was generally less at pH 8 than pH 5 but only 3 cases had p <0.05. Adsorption from 11 unaltered urine samples with albumin 5–333 mg/l was <0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100 ml and 2 l collection containers, and to instrument sample cups and showed <1% change in concentration at 5 mg/l and <0.5% change at 20 mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2–28%) was larger than that from urine.
  
  Conclusions
  
  Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.
  
  
• Subjects:
  Adsorption Albumin Adsorption Albumins Albuminuria Article Humans Hydrogen-Ion Concentration Serum Albumin Serum Albumin, Radio-Iodinated Surface-Active Agents Surface Properties Urine Albumin Urine Containers Urine Specimen Collection

  More +
• Source:
  Clin Chim Acta. 431:40-45.
• Pubmed ID:
  24513540
• Pubmed Central ID:
  PMC5708857
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  431
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b6ad807d4a373cc22078f63fa0d76387770aa9ff0b88cbcaf9e8316a1a17c637
• Download URL:
  https://stacks.cdc.gov/view/cdc/50419/cdc_50419_DS1.pdf
• File Type:
  [PDF - 440.67 KB ]