Effects of preanalytical factors on hemoglobin measurement: a comparison of two HemoCue® point-of-care analyzers

Details

• Alternative Title:
  Clin Biochem
• Personal Author:
  Whitehead, Ralph D. ; Zhang, Ming ; Sternberg, Maya R. ; Schleicher, Rosemary L. ; Drammeh, Bakary ; Mapango, Carine ; Pfeiffer, Christine M.
• Description:
  Background
  
  In field studies, hemoglobin (Hb) is often measured using a battery-operated, portable HemoCue® hemoglobinometer.
  
  Methods
  
  We compared the performance of 2 HemoCue® models (Hb-201+ and Hb-301) and investigated effects of preanalytical factors on Hb results by simulating unfavorable field conditions.
  
  Results
  
  The Hb-301 produced 2.6% higher results compared to the Hb-201+. Hb had to be measured within 1 min of filling the Hb-301 cuvette to avoid artificially elevated concentrations (1.3% per min). The Hb-301 cuvettes withstood elevated temperature (37°C) and humidity (72%) for 3 wk, while the Hb-201+ cuvettes degraded within 10 min under those conditions. Both cuvette types withstood elevated temperature for 3 wk. Properly-collected venous and capillary blood produced comparable results. Pooled capillary blood produced comparable results to the second and third but not the fourth drop of blood (3.3% lower). Blood could be stored for ≤4 d at 10–30°C before Hb-201+ measurement, but only for 1 d at 10–23°C before Hb-301 measurement (≤1% change in Hb).
  
  Conclusions
  
  Higher Hb results obtained with the Hb-301 may influence the interpretation of anemia prevalence in health surveys. While the Hb-301 performed better in high humidity conditions, the Hb-201+ provided more user flexibility regarding delayed Hb reading.
  
  
• Subjects:
  Adult Anemia Blood Chemical Analysis Female Hemoglobins Humans Male Middle Aged Point-of-Care Testing Reproducibility Of Results
• Source:
  Clin Biochem. 50(9):513-520.
• Pubmed ID:
  28412284
• Pubmed Central ID:
  PMC5685159
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  50
• Issue:
  9
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:db2114e7f12894e0ff20ac37f09aace0d030347a1a1de63d72c7074eb47f4b19
• Download URL:
  https://stacks.cdc.gov/view/cdc/49835/cdc_49835_DS1.pdf
• File Type:
  [PDF - 346.30 KB ]