Development and Validation of Benomyl Birdseed Agar for the Isolation of Cryptococcus neoformans and Cryptococcus gattii from Environmental Samples

Details

• Alternative Title:
  Med Mycol
• Personal Author:
  Pham, Cau D. ; Ahn, Stacey ; Turner, Lance A. ; Wohrle, Ron ; Lockhart, Shawn R.
• Description:
  One of the difficulties of isolating Cryptococcus neoformans and Cryptococcus gattii from environmental samples is the abundant overgrowth of other yeast and mold species that occurs on the plates. Here we report the application of benomyl to Guizotia abyssinica seed extract growth medium to improve the isolation of C. neoformans and C. gattii from environmental samples. We validated this medium by recovering C. neoformans and C. gattii from convenience soils and swabs from a region of the United States where these yeasts are endemic.
• Subjects:
  Agar Article Asteraceae Benomyl Birdseed Agar Cryptococcus Gattii Cryptococcus Neoformans Culture Media Environmental Microbiology Plant Extracts Seeds United States

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• Source:
  Med Mycol. 52(4):417-421
• Pubmed ID:
  24782104
• Pubmed Central ID:
  PMC5672335
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  52
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:657f100cd00e210afa9f3753f6300a9cdad13c380c89fde4305d85cbed51955c
• Download URL:
  https://stacks.cdc.gov/view/cdc/49639/cdc_49639_DS1.pdf
• File Type:
  [PDF - 230.03 KB ]