Details:

Alternative Title:
Ticks Tick Borne Dis
Publisher's site:
http://dx.doi.org/10.1016/j.ttbdis.2016.05.011
Personal Author:
Levin, Michael L. ; Snellgrove, Alyssa N. ; Zemtsova, Galina E. ;
Levin, Michael L. ; Snellgrove, Alyssa N. ; Zemtsova, Galina E. ; Less -
Description:
The definitive diagnosis of spotted fever group (SFG) rickettsioses in humans is challenging due to the retrospective nature and cross reactivity of the serological methods and the absence of reliable and consistent samples for molecular diagnostics. Existing data indicate the transient character of bacteremia in experimentally infected animals. The ability of arthropod vectors to acquire rickettsial infection from the laboratory animals in the absence of systemic infection and known tropism of rickettsial agents to endothelial cells of peripheral blood vessels underline the importance of local infection and consequently the diagnostic potential of skin samples. In order to evaluate the diagnostic sensitivity of rickettsial DNA detection in blood and skin samples, we compared results of PCR testing in parallel samples collected from model laboratory animals infected with Rickettsia rickettsii, Rickettsia parkeri and Rickettsia slovaca-like agent at different time points after infection. Skin samples were collected from ears - away from the site of tick placement and without eschars. Overall, testing of skin samples resulted in a higher proportion of positive results than testing of blood samples. Presented data from model animals demonstrates that testing of skin samples from sites of rickettsial proliferation can provide definitive molecular diagnosis of up to 60-70% of tick-borne SFG rickettsial infections during the acute stage of illness. Detection of pathogen DNA in cutaneous samples is a valuable alternative to blood-PCR at least in model animals.
Subject:
[+]
Animals
Arthropod Vectors
Article
Blood
Diagnosis
Disease Models, Animal
DNA, Bacterial
Guinea Pig
Ixodidae
Molecular Diagnostic Techniques
Polymerase Chain Reaction
Retrospective Studies
Rickettsia
Rickettsia Infections
Rickettsia Rickettsii
Rocky Mountain Spotted Fever
Skin
Skin Biopsies
Spotted Fever Rickettsiosis
Tick-Borne Diseases

Source:
Ticks Tick Borne Dis. 7(5):1029-1034.
Pubmed ID:
27282078
Pubmed Central ID:
PMC5661872
Document Type:
Journal Article ;
Funding:
CC999999/Intramural CDC HHS/United States ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:b71341474892bdb5cdd590b294b2c0920424118c10e9d08a867541900b738b51
File Type:

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