Laboratory Diagnosis of Chikungunya Virus Infections and Commercial Sources for Diagnostic Assays

Details

• Alternative Title:
  J Infect Dis
• Personal Author:
  Johnson, Barbara W. ; Russell, Brandy J. ; Goodman, Christin H.
• Description:
  Detection of chikungunya virus (CHIKV) or viral RNA is the primary laboratory test used to diagnose infection in serum collected <6 days after onset of illness. Two real-time reverse transcription-polymerase chain reaction (RT-PCR) kits are available commercially, but validity data are limited. There are 2 commercial sources of inactivated positive-control CHIKV RNA to be used with purchased primers. The Centers for Disease Control and Prevention provides viral RNA-positive controls and primer and probe nucleotide sequences for real-time RT-PCR testing. Detection of CHIKV-specific immunoglobulin M (IgM) antibody becomes a sensitive test for samples collected approximately >5 days of illness. Commercially available CHIKV IgM-detection assays include lateral flow rapid tests, IgM antibody capture enzyme-linked immunosorbent assays (MAC-ELISAs), and indirect immunofluorescence tests. Nine commercial CHIKV IgM detection assays were evaluated at 3 reference laboratories to provide guidance to public health diagnostic laboratories on their performance parameters. Sensitivity of the rapid tests and 3 MAC-ELISAs was <50%, and thus these assays are not recommended. Three of the MAC-ELISA kits and 1 indirect immunofluorescence kit had comparable performance to the reference assays. In summary, commercial assays with performance comparable to reference assays are available for molecular and serological diagnosis of CHIKV infections.
• Subjects:
  Antibodies, Viral Arbovirus Diagnostic Testing Article Chikungunya Fever Chikungunya Virus Clinical Laboratory Techniques Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique, Indirect Fluoroimmunoassay Humans IgM Antibody Capture Enzyme-linked Immunosorbent Assay (MAC-ELISA) Immunoglobulin M Reagent Kits, Diagnostic Real-Time Polymerase Chain Reaction Real-time RT-PCR Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity And Specificity

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• Source:
  J Infect Dis. 214(Suppl 5):S471-S474
• Document Type:
  Journal Article
• Volume:
  214
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:21ea996ad1588bc91172d34846b510a20895fd759df9512f6c6d49f7675bd3b12a0abdca3dc516823317058d78178172c667b3577b7d6735ab8e885cb9883c97
• Download URL:
  https://stacks.cdc.gov/view/cdc/49354/cdc_49354_DS1.pdf
• File Type:
  [PDF - 1.08 MB ]