Details:

Alternative Title:
J Infect Dis
Personal Author:
Johnson, Barbara W. ; Russell, Brandy J. ; Goodman, Christin H.
Johnson, Barbara W. ; Russell, Brandy J. ; Goodman, Christin H. Less -
Description:
Detection of chikungunya virus (CHIKV) or viral RNA is the primary laboratory test used to diagnose infection in serum collected <6 days after onset of illness. Two real-time reverse transcription-polymerase chain reaction (RT-PCR) kits are available commercially, but validity data are limited. There are 2 commercial sources of inactivated positive-control CHIKV RNA to be used with purchased primers. The Centers for Disease Control and Prevention provides viral RNA-positive controls and primer and probe nucleotide sequences for real-time RT-PCR testing. Detection of CHIKV-specific immunoglobulin M (IgM) antibody becomes a sensitive test for samples collected approximately >5 days of illness. Commercially available CHIKV IgM-detection assays include lateral flow rapid tests, IgM antibody capture enzyme-linked immunosorbent assays (MAC-ELISAs), and indirect immunofluorescence tests. Nine commercial CHIKV IgM detection assays were evaluated at 3 reference laboratories to provide guidance to public health diagnostic laboratories on their performance parameters. Sensitivity of the rapid tests and 3 MAC-ELISAs was <50%, and thus these assays are not recommended. Three of the MAC-ELISA kits and 1 indirect immunofluorescence kit had comparable performance to the reference assays. In summary, commercial assays with performance comparable to reference assays are available for molecular and serological diagnosis of CHIKV infections.
Subjects:
[+]
Antibodies, Viral Arbovirus Diagnostic Testing Article Chikungunya Fever Chikungunya Virus Clinical Laboratory Techniques Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique, Indirect Fluoroimmunoassay Humans IgM Antibody Capture Enzyme-linked Immunosorbent Assay (MAC-ELISA) Immunoglobulin M Reagent Kits, Diagnostic Real-Time Polymerase Chain Reaction Real-time RT-PCR Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity And Specificity
Source:
J Infect Dis. 214(Suppl 5):S471-S474
Pubmed ID:
27920176
Pubmed Central ID:
PMC5657184
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHSUnited States/
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:6a5c116291c2ed76bf610b42e630870d4150eeb345c3fbda5201bf987d96d48d
Download URL:
https://stacks.cdc.gov/view/cdc/49354/cdc_49354_DS1.pdf
File Type:

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