Details:

Alternative Title:
AIDS Res Hum Retroviruses
Personal Author:
Haaland, Richard E. ; Sullivan, Sharon T. ; Evans-Strickfaden, Tammy ; Lennox, Jeffrey L. ; Hart, Clyde E.
Haaland, Richard E. ; Sullivan, Sharon T. ; Evans-Strickfaden, Tammy ; Lennox, Jeffrey L. ; Hart, Clyde E. Less -
Description:
This study compared HIV-1 genotypes shed over time (≤3.5 years) in the vaginal secretions (VS) and blood plasma (BP) of 15 chronically infected women. Analysis of predicted coreceptor tropism (CCR5=R5, CXCR4=X4) for quasispecies shedding revealed three patterns: (1) viral quasispecies shed in both VS and BP were restricted to R5-tropism at all time points, (2) quasispecies shed in VS were restricted to R5-tropism at all time points but X4 quasispecies were identified in the BP at one or more time points, and (3) quasispecies shed in matched VS and BP both contained X4-tropic viruses. Overall, the frequency of X4 quasispecies circulation in VS was 2-fold less than in BP and detection of X4 virus in VS was more likely to occur when X4 quasispecies comprised more than 50% of BP viruses (p=0.01) and when declines in blood CD4(+) lymphocyte levels were the greatest (p=0.038). Additionally, the mean number of predicted N-glycosylation sites between matched VS and BP samples was strongly correlated (r=0.86, p<0.0001) with glycosylation densities in the following order (VS R5=BP R5 > BP X4 > VS X4). The X4 glycosylation densities may result from compartmentalization pressures in the female genital tract or the delayed appearance of these viruses in VS. Our results suggest that the presence of X4 virus in VS is associated with a threshold population of X4 quasispecies in BP, which are increasing during the HIV-induced failure of the human immune system.
Subjects:
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Adult Article CD4 Lymphocyte Count Cell Line Female HIV-1 HIV Envelope Protein Gp120 HIV Infections Humans Longitudinal Studies Middle Aged Polymerase Chain Reaction Receptors, CCR5 Receptors, CXCR4 Vagina Viral Tropism Virus Replication Virus Shedding
Source:
AIDS Res Hum Retroviruses. 28(11):1524-1532
Pubmed ID:
22369497
Pubmed Central ID:
PMC5555632
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHSUnited States/
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:dba0f8e6fc11acaef3a44978908021d107c5ed7b5e3158a0dc84acdbbd1923ae
Download URL:
https://stacks.cdc.gov/view/cdc/47542/cdc_47542_DS1.pdf
File Type:

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