Interleukin (IL)-6 modulates transforming growth factor -β receptor I and II (TGF-βRI and II) function in epidermal keratinocytes

Luckett-Chastain, Lerin R.; Cottrell, Mackenzie L.; Kawar, Bethany M.; Ihnat, Michael A.; Gallucci, Randle M.

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Interleukin (IL)-6 modulates transforming growth factor -β receptor I and II (TGF-βRI and II) function in epidermal keratinocytes

Published Date:

Mar 08 2017

Source:

Exp Dermatol. 26(8):697-704.

Language:

English

[PDF-1.46 MB]

Details:

Alternative Title:

Exp Dermatol

Personal Author:

Luckett-Chastain, Lerin R. ; Cottrell, Mackenzie L. ; Kawar, Bethany M. ; Ihnat, Michael A. ; Gallucci, Randle M. ;

Description:

It been shown that IL-6 modulates TGF-β1 expression in fibroblasts, however, what role IL-6 plays concerning TGF-βR expression and function in skin is unknown. Therefore, the aim of this study was to investigate the mechanism by which IL-6 might modulates TGF-β receptors in skin. Skin from WT, IL-6 over-expressing mice and IL-6 treated keratinocyte cultures was analysed for TGF-βRI and TGF-βRII expression via histology, PCR and flow cytometry. Receptor function was assessed by cell migration, bromodeoxyuridine (BrdU) proliferation assays, and Smad7 expression and Smad2/3 phosphorylation. Receptor localization within the membrane was determined by co-immunoprecipitation. IL-6 overexpression and treatment increased TGF-βRII expression in the epidermis. IL-6 treatment of keratinocytes induced TGF-βRI and II expression and augmented TGF-β1-induced function as demonstrated through increased migration and decreased proliferation. Additionally, IL-6 treatment of keratinocytes altered receptor activity as indicated by altered Smad2/3 phosphorylation and increased Smad7 and membrane localization. These results suggest that IL-6 regulates keratinocyte function by modulating TGF-βRI and II expression and signal transduction via trafficking of the receptor to lipid raft pools.

Subject:

[+]

Pubmed ID:

27892604

Pubmed Central ID:

PMC5446936

Document Type:

Journal Article

Funding:

R01 GM067745/GM/NIGMS NIH HHS/United States ; R01 OH010241/OH/NIOSH CDC HHS/United States ;

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	nihms-832517.nxml	text/xml
	nihms-832517-f0001.gif	image/gif
	nihms-832517-f0001.jpg	image/jpeg
	nihms-832517-f0002.gif	image/gif
	nihms-832517-f0002.jpg	image/jpeg
	nihms-832517-f0003.gif	image/gif
	nihms-832517-f0003.jpg	image/jpeg
	nihms-832517-f0004.gif	image/gif
	nihms-832517-f0004.jpg	image/jpeg

No Related Documents.

You May Also Like:

Pulmonary toxicity and fibrogenic response of carbon nanotubes

Personal Author:

Manke, Amruta; Wang, Liying; Rojanasakul, Yon;

Published Date:

Jan 16 2013

Source:

Toxicol Mech Methods. 23(3):196-206.

Description:

Carbon nanotubes (CNTs) have been a subject of intensive research for a wide range of applications. However, because of their extremely small size and light weight, CNTs are readily inhaled into human lungs resulting in increased rates of pulmonary d...

File Type:

[PDF - 314.45 KB]
Advances in mechanisms and signaling pathways of carbon nanotube toxicity

Personal Author:

Dong, Jie; Ma, Qiang;

Published Date:

Feb 13 2015

Source:

Nanotoxicology. 9(5):658-676.

Description:

Carbon nanotubes (CNT) have been developed into new materials with a variety of industrial and commercial applications. In contrast, the physicochemical properties of CNT at the nanoscale render them the potency to generate toxic effects. Indeed, the...

File Type:

[PDF - 1.76 MB]
Myofibroblasts and lung fibrosis induced by carbon nanotube exposure

Personal Author:

Dong, Jie; Ma, Qiang;

Published Date:

Nov 04 2016

Source:

Part Fibre Toxicol. 13.

Description:

Carbon nanotubes (CNTs) are newly developed materials with unique properties and a range of industrial and commercial applications. A rapid expansion in the production of CNT materials may increase the risk of human exposure to CNTs. Studies in roden...

File Type:

[PDF - 1.94 MB]
MicroRNA-26a Is a Novel Regulator of Vascular Smooth Muscle Cell Function

Personal Author:

Leeper, Nicholas J.; Raiesdana, Azad; Kojima, Yoko; Chun, Hyung J.; Azuma, Junya; Maegdefessel, Lars; Kundu, Ramendra K.; Quertermous, Thomas; Tsao, Philip S.; Spin, Joshua M.;

Published Date:

Apr 2011

Source:

J Cell Physiol. 2010; 226(4):1035-1043.

Description:

Aberrant smooth muscle cell (SMC) plasticity has been implicated in a variety of vascular disorders including atherosclerosis, restenosis, and abdominal aortic aneurysm (AAA) formation. While the pathways governing this process remain unclear, epigen...

File Type:

[PDF - 2.83 MB]
Asbestos-associated mesothelial cell autoantibodies promote collagen deposition in vitro

Personal Author:

Serve, Kinta M.; Black, Brad; Szeinuk, Jaime; Pfau, Jean C.;

Published Date:

Dec 2013

Source:

Inhal Toxicol. 25(14):774-784.

Description:

Fibrosis, characterized by excessive collagen protein deposition, is a progressive disease that can fatally inhibit organ function. Prolonged exposure to pathogens or environmental toxicants such as asbestos can lead to chronic inflammatory responses...

File Type:

[PDF - 854.53 KB]