Details:

Alternative Title:
Exp Dermatol
Personal Author:
Luckett-Chastain, Lerin R. ; Cottrell, Mackenzie L. ; Kawar, Bethany M. ; Ihnat, Michael A. ; Gallucci, Randle M.
Luckett-Chastain, Lerin R. ; Cottrell, Mackenzie L. ; Kawar, Bethany M. ; Ihnat, Michael A. ; Gallucci, Randle M. Less -
Description:
It been shown that IL-6 modulates TGF-β1 expression in fibroblasts, however, what role IL-6 plays concerning TGF-βR expression and function in skin is unknown. Therefore, the aim of this study was to investigate the mechanism by which IL-6 might modulates TGF-β receptors in skin. Skin from WT, IL-6 over-expressing mice and IL-6 treated keratinocyte cultures was analysed for TGF-βRI and TGF-βRII expression via histology, PCR and flow cytometry. Receptor function was assessed by cell migration, bromodeoxyuridine (BrdU) proliferation assays, and Smad7 expression and Smad2/3 phosphorylation. Receptor localization within the membrane was determined by co-immunoprecipitation. IL-6 overexpression and treatment increased TGF-βRII expression in the epidermis. IL-6 treatment of keratinocytes induced TGF-βRI and II expression and augmented TGF-β1-induced function as demonstrated through increased migration and decreased proliferation. Additionally, IL-6 treatment of keratinocytes altered receptor activity as indicated by altered Smad2/3 phosphorylation and increased Smad7 and membrane localization. These results suggest that IL-6 regulates keratinocyte function by modulating TGF-βRI and II expression and signal transduction via trafficking of the receptor to lipid raft pools.
Subject:
[+]
Animals
Animals, Newborn
Article
Caveolin
Gene Knockout Techniques
Interleukin-6
Keratinocytes
Mice
Migration
Protein-Serine-Threonine Kinases
Receptor Endocytosis
Receptors, Transforming Growth Factor Beta
Skin
Smad
Smad Proteins

Source:
Exp Dermatol. 26(8):697-704.
Pubmed ID:
27892604
Pubmed Central ID:
PMC5446936
Document Type:
Journal Article
Funding:
R01 GM067745/GM/NIGMS NIH HHS/United States ; R01 OH010241/OH/NIOSH CDC HHS/United States
R01 GM067745/GM/NIGMS NIH HHS/United States ; R01 OH010241/OH/NIOSH CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:aa592aca92844424315c59b0f8e83d968e5d6dd834c3804326f25fb87f583f88
File Type:

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