Details:

Alternative Title:
Toxicon
Personal Author:
Schieltz, David M. ; McWilliams, Lisa G. ; Kuklenyik, Zsuzsanna ; Prezioso, Samantha M. ; Carter, Andrew J. ; ... More +
Schieltz, David M. ; McWilliams, Lisa G. ; Kuklenyik, Zsuzsanna ; Prezioso, Samantha M. ; Carter, Andrew J. ; Williamson, Yulanda M. ; McGrath, Sara C. ; Morse, Stephen A. ; Barr, John R. Less -
Description:
The seeds of the Ricinus communis (Castor bean) plant are the source of the economically important commodity castor oil. Castor seeds also contain the proteins ricin and R. communis agglutinin (RCA), two toxic lectins that are hazardous to human health. Radial immunodiffusion (RID) and the enzyme linked immunosorbent assay (ELISA) are two antibody-based methods commonly used to quantify ricin and RCA; however, antibodies currently used in these methods cannot distinguish between ricin and RCA due to the high sequence homology of the respective proteins. In this study, a technique combining antibody-based affinity capture with liquid chromatography and multiple reaction monitoring (MRM) mass spectrometry (MS) was used to quantify the amounts of ricin and RCA independently in extracts prepared from the seeds of eighteen representative cultivars of R. communis which were propagated under identical conditions. Additionally, liquid chromatography and MRM-MS was used to determine rRNA N-glycosidase activity for each cultivar and the overall activity in these cultivars was compared to a purified ricin standard. Of the cultivars studied, the average ricin content was 9.3 mg/g seed, the average RCA content was 9.9 mg/g seed, and the enzymatic activity agreed with the activity of a purified ricin reference within 35% relative activity.
Subject:
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Article
Castor Bean
Chromatography, Liquid
Cultivar
Electrophoresis, Polyacrylamide Gel
Glycoside Hydrolases
Immunoassay
Mass Spectrometry
Plant Extracts
Plant Lectins
Quantification
RCA
Ricin
Ricinus Communis
Seeds
Tandem Mass Spectrometry

Source:
Toxicon. 95:72-83.
Pubmed ID:
25576235
Pubmed Central ID:
PMC5303535
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:9666b4882571e49efe1a194e5f515f49df9a68259d36b8bc78fc1ba167bdb13c
File Type:

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