Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load☆

Mijatovic-Rustempasic, Slavica; Esona, Mathew D.; Williams, Alice L.; Bowen, Michael D.

doi:10.1016/j.jviromet.2016.07.007

Back to Previous Page

Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load☆

Published Date:

Jul 13 2016

Publisher's site:

http://dx.doi.org/10.1016/j.jviromet.2016.07.007

Source:

J Virol Methods. 236:41-46.

[PDF-489.24 KB]

Details:

Alternative Title:

J Virol Methods

Personal Author:

Mijatovic-Rustempasic, Slavica ; Esona, Mathew D. ; Williams, Alice L. ; Bowen, Michael D. ;

Description:

Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. The assay developed in this study detects rotavirus G genotypes and P genotypes with viral loads as low as 6.2 and 8.2 copies per reaction, respectively. The assay was validated using a panel of 91 stool samples, 32 reference rotavirus strains, and 6 non-target enteric virus samples.

Subject:

[+]

Pubmed ID:

27421626

Pubmed Central ID:

PMC5075964

Document Type:

Journal Article

Funding:

CC999999/Intramural CDC HHS/United States

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

File Type:

Supporting Files:

	nihms822984.nxml	application/octet-stream
	nihms822984f1.gif	image/gif
	nihms822984f1.jpg	image/jpeg

No Related Documents.

Comparison of PremierTM Rotaclone®, ProSpecTTM, and RIDASCREEN® Rotavirus Enzyme Immunoassay Kits for Detection of Rotavirus Antigen in Stool Specimens

[PDF - 224.05 KB]

Personal Author:

Gautam, Rashi ; Lyde, Freda ; ...

Published Date:

Jul 10 2013

Source:

J Clin Virol. 58(1):292-294.

Description:

BackgroundRotaviruses are the major cause of severe dehydrating diarrhea in children throughout the world. Enzyme immunoassays (EIA) have been the sta...

File Type:

[PDF - 224.05 KB]

File Type:

[PDF - 224.05 KB]
One Year Survey of Human Rotavirus Strains Suggests the Emergence of Genotype G12 in Cameroon

[PDF - 471.82 KB]

Personal Author:

Ndze, Valentine N. ; Papp, Hajnalka ; ...

Published Date:

2013

Source:

J Med Virol. 85(8):1485-1490

Description:

In this study the emergence of rotavirus A genotype G12 in children <5 years of age is reported from Cameroon during 2010/2011. A total of 135 human s...

File Type:

[PDF - 471.82 KB]

File Type:

[PDF - 471.82 KB]
SARS Coronavirus Detection

[PDF - 185.56 KB]

Personal Author:

Nitsche, Andreas ; Schweiger, Brunhilde ; ...

Published Date:

Jul 2004

Source:

Emerg Infect Dis. 10(7):1300-1303.

Description:

We developed a set of three real-time reverse transcription-polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-as...

File Type:

[PDF - 185.56 KB]

File Type:

[PDF - 185.56 KB]
Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients

[PDF - 309.43 KB]

Personal Author:

Wölfel, Roman ; Paweska, Janusz T. ; ...

Published Date:

Jul 2007

Source:

Emerg Infect Dis. 13(7):1097-1100.

Description:

We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at ...

File Type:

[PDF - 309.43 KB]

File Type:

[PDF - 309.43 KB]
Influenza A H5N1 Detection

[PDF - 95.13 KB]

Personal Author:

Ng, Enders K.O. ; Cheng, Peter K.C. ; ...

Published Date:

Aug 2005

Source:

Emerg Infect Dis. 11(8):1303-1305.

Description:

We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinic...

File Type:

[PDF - 95.13 KB]

File Type:

[PDF - 95.13 KB]

Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load☆

Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load☆

You May Also Like:

Have Questions?

CDC INFORMATION

CONNECT WITH CDC