Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load☆

Mijatovic-Rustempasic, Slavica; Esona, Mathew D.; Williams, Alice L.; Bowen, Michael D.

doi:10.1016/j.jviromet.2016.07.007

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Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load☆

Jul 13 2016
By Mijatovic-Rustempasic, Slavica ; Esona, Mathew D. ; Williams, Alice L. ; ...
http://dx.doi.org/10.1016/j.jviromet.2016.07.007
Source: J Virol Methods. 236:41-46.

[PDF-489.24 KB]

English

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Details:

Alternative Title:

J Virol Methods
Personal Author:

Mijatovic-Rustempasic, Slavica ; Esona, Mathew D. ; Williams, Alice L. ; Bowen, Michael D.

Mijatovic-Rustempasic, Slavica ; Esona, Mathew D. ; Williams, Alice L. ; Bowen, Michael D. Less -
Description:

Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. The assay developed in this study detects rotavirus G genotypes and P genotypes with viral loads as low as 6.2 and 8.2 copies per reaction, respectively. The assay was validated using a panel of 91 stool samples, 32 reference rotavirus strains, and 6 non-target enteric virus samples.
Subjects:

[+]

Feces Humans Polymerase Chain Reaction Rotavirus Rotavirus Infections Sensitivity And Specificity
Source:

J Virol Methods. 236:41-46.
Pubmed ID:

27421626
Pubmed Central ID:

PMC5075964
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHS/United States
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:73d99068a65e18e0b5e6bfcb17e774f66c41be4193c80a40f8a6692f49487987
Download URL:

https://stacks.cdc.gov/view/cdc/42222/cdc_42222_DS1.pdf
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	nihms822984.nxml	bin
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