Metabolism of the Antibacterial Triclocarban by Human Epidermal Keratinocytes to Yield Protein Adducts

Details

• Alternative Title:
  J Biochem Mol Toxicol
• Personal Author:
  Schebb, Nils Helge ; Buchholz, Bruce A. ; Hammock, Bruce D. ; Rice, Robert H.
• Description:
  Previous studies of triclocarban suggest that its biotransformation could yield reactive metabolites that form protein adducts. Since the skin is the major route of triclocarban exposure, present work examined this possibility in cultured human keratinocytes. The results provide evidence for considerable biotransformation and protein adduct formation when cytochrome P450 activity is induced in the cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin, a model Ah receptor ligand. Since detecting low adduct levels in cells and tissues is difficult, we utilized the novel approach of accelerator mass spectrometry for this purpose. Exploiting the sensitivity of the method, we demonstrated that a substantial portion of triclocarban forms adducts with keratinocyte protein under the P450 inducing conditions employed.
• Subjects:
  Anti-Bacterial Agents Carbanilides Cytochrome P-450 Enzyme System Epidermis Humans Proteins Tetrachlorodibenzodioxin
• Source:
  J Biochem Mol Toxicol. 26(6):230-234.
• Pubmed ID:
  22711420
• Pubmed Central ID:
  PMC3522462
• Document Type:
  Journal Article
• Funding:
  U50 OH007550/OH/NIOSH CDC HHS/United States ; P41 RR013461/RR/NCRR NIH HHS/United States ; R01 ES002710/ES/NIEHS NIH HHS/United States ; P42 ES004699/ES/NIEHS NIH HHS/United States ; RR13461/RR/NCRR NIH HHS/United States ; P41 GM103483/GM/NIGMS NIH HHS/United States ; 8 P41 GM103483-14/GM/NIGMS NIH HHS/United States ; U54 OH007550/OH/NIOSH CDC HHS/United States
• Volume:
  26
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ae736e25b0e418ad97d54fbe64ce2799cfb1d1572376777c7c6c79d9f4d1be28
• Download URL:
  https://stacks.cdc.gov/view/cdc/41871/cdc_41871_DS1.pdf
• File Type:
  [PDF - 397.33 KB ]