Interrogating cellular fate decisions with high-throughput arrays of multiplexed cellular communities

Chen, Sisi; Bremer, Andrew W.; Scheideler, Olivia J.; Na, Yun Suk; Todhunter, Michael E.; Hsiao, Sonny; Bomdica, Prithvi R.; Maharbiz, Michel M.; Gartner, Zev J.; Schaffer, David V.

doi:10.1038/ncomms10309

i

Interrogating cellular fate decisions with high-throughput arrays of multiplexed cellular communities

Supporting Files

Jan 12 2016
By Chen, Sisi ; Bremer, Andrew W. ; Scheideler, Olivia J. ; ...

File Language:

English

Details

Alternative Title:

Nat Commun
Personal Author:

Chen, Sisi ; Bremer, Andrew W. ; Scheideler, Olivia J. ; Na, Yun Suk ; Todhunter, Michael E. ; Hsiao, Sonny ; Bomdica, Prithvi R. ; Maharbiz, Michel M. ; Gartner, Zev J. ; Schaffer, David V.
Description:

Recreating heterotypic cell-cell interactions in vitro is key to dissecting the role of cellular communication during a variety of biological processes. This is especially relevant for stem cell niches, where neighbouring cells provide instructive inputs that govern cell fate decisions. To investigate the logic and dynamics of cell-cell signalling networks, we prepared heterotypic cell-cell interaction arrays using DNA-programmed adhesion. Our platform specifies the number and initial position of up to four distinct cell types within each array and offers tunable control over cell-contact time during long-term culture. Here, we use the platform to study the dynamics of single adult neural stem cell fate decisions in response to competing juxtacrine signals. Our results suggest a potential signalling hierarchy between Delta-like 1 and ephrin-B2 ligands, as neural stem cells adopt the Delta-like 1 phenotype of stem cell maintenance on simultaneous presentation of both signals.
Subjects:

Animals Astrocytes Cell Communication Cell Differentiation Cell Line Ephrin-B2 HEK293 Cells High-Throughput Screening Assays Humans Intercellular Signaling Peptides And Proteins Membrane Proteins Neural Stem Cells Paracrine Communication Phenotype Rats Time-Lapse Imaging Tissue Array Analysis
Source:

Nat Commun. 2016; 7.
Pubmed ID:

26754526
Pubmed Central ID:

PMC4729920
Document Type:

Journal Article
Funding:

DP2 HD080351-01/DP/NCCDPHP CDC HHS/United States ; R01 ES020903/ES/NIEHS NIH HHS/United States ; R21 EB014610/EB/NIBIB NIH HHS/United States
Volume:

7
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:bbefa8960411bb87db59a593ef580282ec4ff2a5f578bfec81ecb61595bd23c8
Download URL:

https://stacks.cdc.gov/view/cdc/41033/cdc_41033_DS1.pdf
File Type:

[PDF - 2.13 MB ]

File Language:

English

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