Immunohistochemical detection of markers for translational studies of lung disease in pigs and humans

Meyerholz, David K.; Lambertz, Allyn M.; Reznikov, Leah R.; Ofori-Amanfo, Georgina K.; Karp, Phil H.; McCray, Paul B.; Welsh, Michael J.; Stoltz, David A.

doi:10.1177/0192623315609691

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Immunohistochemical detection of markers for translational studies of lung disease in pigs and humans

Oct 27 2015
By Meyerholz, David K. ; Lambertz, Allyn M. ; Reznikov, Leah R. ; ...
http://dx.doi.org/10.1177/0192623315609691
Source: Toxicol Pathol. 44(3):434-441.

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Details:

Alternative Title:

Toxicol Pathol
Personal Author:

Meyerholz, David K. ; Lambertz, Allyn M. ; Reznikov, Leah R. ; Ofori-Amanfo, Georgina K. ; Karp, Phil H. ; ... More +

Meyerholz, David K. ; Lambertz, Allyn M. ; Reznikov, Leah R. ; Ofori-Amanfo, Georgina K. ; Karp, Phil H. ; McCray, Paul B. ; Welsh, Michael J. ; Stoltz, David A. Less -
Description:

Genetically engineered pigs are increasingly recognized as valuable models for the study of human disease. Immunohistochemical study of cellular markers of disease is an important tool for the investigation of these novel models so as to evaluate genotype and treatment differences. Even so, there remains a lack of validated markers for pig tissues that can serve as a translational link to human disease in organs such as the lung. Herein, we evaluate markers of cellular inflammation (cluster of differentiation [CD]3, CD79a, B cell lymphoma [BCL] 6, ionized calcium-binding adapter molecule [IBA]1, and myeloperoxidase) and those that may be involved with tissue remodeling (alpha-smooth muscle actin, beta-tubulin-III, lactoferrin, mucin [MUC]5AC, MUC5B, and cystic fibrosis transmembrane conductance regulator [CFTR]) for study of lung tissues. We compare the utility of these markers between pig and human lungs to validate translational relevance of each marker. Our results suggest these markers can be a useful addition in the pathological evaluation of porcine models of human disease.
Subjects:

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Animals Article Biomarkers Disease Models, Animal Human Humans Immunohistochemistry Leukocytes Lung Markers Mucus Pig Pneumonia Reproducibility Of Results Swine
Source:

Toxicol Pathol. 44(3):434-441.
Pubmed ID:

26511846
Pubmed Central ID:

PMC4805467
Document Type:

Journal Article
Funding:

DP2HL117744/DP/NCCDPHP CDC HHS/United States ; P01 HL091842/HL/NHLBI NIH HHS/United States ; P01HL051670/HL/NHLBI NIH HHS/United States ; P01 HL051670/HL/NHLBI NIH HHS/United States ; P30 ES005605/ES/NIEHS NIH HHS/United States ; ... More +

DP2HL117744/DP/NCCDPHP CDC HHS/United States ; P01 HL091842/HL/NHLBI NIH HHS/United States ; P01HL051670/HL/NHLBI NIH HHS/United States ; P01 HL051670/HL/NHLBI NIH HHS/United States ; P30 ES005605/ES/NIEHS NIH HHS/United States ; Howard Hughes Medical Institute/United States ; P30 DK054759/DK/NIDDK NIH HHS/United States ; K99 HL119560/HL/NHLBI NIH HHS/United States ; 1K99HL119560/HL/NHLBI NIH HHS/United States ; DP2 HL117744/HL/NHLBI NIH HHS/United States Less -
Volume:

44
Issue:

3
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:b1dad2c667d0eff8936f17a44cd40a68fa819a94b8495a10431b6bfc03da77d3
Download URL:

https://stacks.cdc.gov/view/cdc/40480/cdc_40480_DS1.pdf
File Type:

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