Immunohistochemical detection of markers for translational studies of lung disease in pigs and humans

Details

• Alternative Title:
  Toxicol Pathol
• Personal Author:
  Meyerholz, David K. ; Lambertz, Allyn M. ; Reznikov, Leah R. ; Ofori-Amanfo, Georgina K. ; Karp, Phil H. ; McCray, Paul B. ; Welsh, Michael J. ; Stoltz, David A.
• Description:
  Genetically engineered pigs are increasingly recognized as valuable models for the study of human disease. Immunohistochemical study of cellular markers of disease is an important tool for the investigation of these novel models so as to evaluate genotype and treatment differences. Even so, there remains a lack of validated markers for pig tissues that can serve as a translational link to human disease in organs such as the lung. Herein, we evaluate markers of cellular inflammation (cluster of differentiation [CD]3, CD79a, B cell lymphoma [BCL] 6, ionized calcium-binding adapter molecule [IBA]1, and myeloperoxidase) and those that may be involved with tissue remodeling (alpha-smooth muscle actin, beta-tubulin-III, lactoferrin, mucin [MUC]5AC, MUC5B, and cystic fibrosis transmembrane conductance regulator [CFTR]) for study of lung tissues. We compare the utility of these markers between pig and human lungs to validate translational relevance of each marker. Our results suggest these markers can be a useful addition in the pathological evaluation of porcine models of human disease.
• Subjects:
  Animals Article Biomarkers Disease Models, Animal Human Humans Immunohistochemistry Leukocytes Lung Markers Mucus Pig Pneumonia Reproducibility Of Results Swine

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• Source:
  Toxicol Pathol. 44(3):434-441.
• Pubmed ID:
  26511846
• Pubmed Central ID:
  PMC4805467
• Document Type:
  Journal Article
• Funding:
  DP2HL117744/DP/NCCDPHP CDC HHS/United States ; P01 HL091842/HL/NHLBI NIH HHS/United States ; P01HL051670/HL/NHLBI NIH HHS/United States ; P01 HL051670/HL/NHLBI NIH HHS/United States ; P30 ES005605/ES/NIEHS NIH HHS/United States ; Howard Hughes Medical Institute/United States ; P30 DK054759/DK/NIDDK NIH HHS/United States ; K99 HL119560/HL/NHLBI NIH HHS/United States ; 1K99HL119560/HL/NHLBI NIH HHS/United States ; DP2 HL117744/HL/NHLBI NIH HHS/United States
• Volume:
  44
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b1dad2c667d0eff8936f17a44cd40a68fa819a94b8495a10431b6bfc03da77d3
• Download URL:
  https://stacks.cdc.gov/view/cdc/40480/cdc_40480_DS1.pdf
• File Type:
  [PDF - 5.32 MB ]