Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy
Published Date:Mar 1 2016
Source:Opt Lett. 41(5):855-858.
Pubmed Central ID:PMC4894304
Funding:DP1 EY024503/EY/NEI NIH HHS/United States
DP1EY024503/DP/NCCDPHP CDC HHS/United States
MH100561/MH/NIMH NIH HHS/United States
MH101218/MH/NIMH NIH HHS/United States
R01 EY011787/EY/NEI NIH HHS/United States
R01 MH101218/MH/NIMH NIH HHS/United States
R01EY011787/EY/NEI NIH HHS/United States
T32EY013933/EY/NEI NIH HHS/United States
Howard Hughes Medical Institute/United States
Description:Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160 μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.
application/octet-stream image/gif image/jpeg image/gif image/jpeg image/gif image/jpeg image/gif image/jpeg
You May Also Like: