Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Quirin, Sean ; Vladimirov, Nikita ; Yang, Chao-Tsung ; Peterka, Darcy S. ; Yuste, Rafael ; Ahrens, Misha B.
• Description:
  Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.
• Subjects:
  Animals Brain Caenorhabditis Elegans Calcium Light Microscopy Molecular Imaging Neurons
• Source:
  Opt Lett. 41(5):855-858
• Pubmed ID:
  26974063
• Pubmed Central ID:
  PMC4894304
• Document Type:
  Journal Article
• Funding:
  R01 MH100561/MH/NIMH NIH HHSUnited States/ ; R01 MH101218/MH/NIMH NIH HHSUnited States/ ; DP1 EY024503/EY/NEI NIH HHSUnited States/ ; MH101218/MH/NIMH NIH HHSUnited States/ ; T32EY013933/EY/NEI NIH HHSUnited States/ ; R01 EY011787/EY/NEI NIH HHSUnited States/ ; Howard Hughes Medical InstituteUnited States/ ; DP1EY024503/DP/NCCDPHP CDC HHSUnited States/ ; MH100561/MH/NIMH NIH HHSUnited States/ ; R01EY011787/EY/NEI NIH HHSUnited States/ ; T32 EY013933/EY/NEI NIH HHSUnited States/
• Volume:
  41
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:182c907f45c11a1cbc77d5e0d8741f62f998cb16d5cb81bd352bb1a63f9b4ca4
• Download URL:
  https://stacks.cdc.gov/view/cdc/40283/cdc_40283_DS1.pdf
• File Type:
  [PDF - 1.26 MB ]