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Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy
  • Published Date:
    Mar 1 2016
  • Source:
    Opt Lett. 41(5):855-858.
Filetype[PDF-1.26 MB]

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  • Description:
    Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.

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  • Funding:
    DP1 EY024503/EY/NEI NIH HHS/United States
    DP1EY024503/DP/NCCDPHP CDC HHS/United States
    MH100561/MH/NIMH NIH HHS/United States
    MH101218/MH/NIMH NIH HHS/United States
    R01 EY011787/EY/NEI NIH HHS/United States
    R01 MH101218/MH/NIMH NIH HHS/United States
    R01EY011787/EY/NEI NIH HHS/United States
    T32EY013933/EY/NEI NIH HHS/United States
    Howard Hughes Medical Institute/United States
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