Welcome to CDC Stacks | Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy - 40283 | CDC Public Access
Stacks Logo
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.
 
 
Help
Clear All Simple Search
Advanced Search
Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy
  • Published Date:
    Mar 1 2016
  • Source:
    Opt Lett. 41(5):855-858.
Filetype[PDF - 1.26 MB]


Details:
  • Pubmed ID:
    26974063
  • Pubmed Central ID:
    PMC4894304
  • Description:
    Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.

  • Document Type:
  • Collection(s):
  • Funding:
    DP1 EY024503/EY/NEI NIH HHS/United States
    DP1EY024503/DP/NCCDPHP CDC HHS/United States
    MH100561/MH/NIMH NIH HHS/United States
    MH101218/MH/NIMH NIH HHS/United States
    R01 EY011787/EY/NEI NIH HHS/United States
    R01 MH101218/MH/NIMH NIH HHS/United States
    R01EY011787/EY/NEI NIH HHS/United States
    T32EY013933/EY/NEI NIH HHS/United States
    Howard Hughes Medical Institute/United States
No Related Documents.
You May Also Like: