Details:

Alternative Title:
Opt Lett
Personal Author:
Quirin, Sean ; Vladimirov, Nikita ; Yang, Chao-Tsung ; Peterka, Darcy S. ; Yuste, Rafael ; ... More +
Quirin, Sean ; Vladimirov, Nikita ; Yang, Chao-Tsung ; Peterka, Darcy S. ; Yuste, Rafael ; Ahrens, Misha B. Less -
Description:
Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.
Subject:
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Animals
Article
Brain
Caenorhabditis Elegans
Calcium
Light
Microscopy
Molecular Imaging
Neurons

Source:
Opt Lett. 41(5):855-858.
Pubmed ID:
26974063
Pubmed Central ID:
PMC4894304
Document Type:
Journal Article
Funding:
R01 MH100561/MH/NIMH NIH HHS/United States ; R01 MH101218/MH/NIMH NIH HHS/United States ; DP1 EY024503/EY/NEI NIH HHS/United States ; MH101218/MH/NIMH NIH HHS/United States ; T32EY013933/EY/NEI NIH HHS/United States ; ... More +
R01 MH100561/MH/NIMH NIH HHS/United States ; R01 MH101218/MH/NIMH NIH HHS/United States ; DP1 EY024503/EY/NEI NIH HHS/United States ; MH101218/MH/NIMH NIH HHS/United States ; T32EY013933/EY/NEI NIH HHS/United States ; R01 EY011787/EY/NEI NIH HHS/United States ; Howard Hughes Medical Institute/United States ; DP1EY024503/DP/NCCDPHP CDC HHS/United States ; MH100561/MH/NIMH NIH HHS/United States ; R01EY011787/EY/NEI NIH HHS/United States ; T32 EY013933/EY/NEI NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:182c907f45c11a1cbc77d5e0d8741f62f998cb16d5cb81bd352bb1a63f9b4ca4
File Type:

Supporting Files

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