Details:

Alternative Title:
Cell Stem Cell
Personal Author:
Ariyachet, Chaiyaboot ; Tovaglieri, Alessio ; Xiang, Guanjue ; Lu, Jiaqi ; Shah, Manasvi S. ; ... More +
Ariyachet, Chaiyaboot ; Tovaglieri, Alessio ; Xiang, Guanjue ; Lu, Jiaqi ; Shah, Manasvi S. ; Richmond, Camilla A. ; Verbeke, Catia ; Melton, Douglas A. ; Stanger, Ben Z. ; Mooney, David ; Shivdasani, Ramesh A. ; Mahony, Shaun ; Xia, Qing ; Breault, David T. ; Zhou, Qiao Less -
Description:
The gastrointestinal (GI) epithelium is a highly regenerative tissue with the potential to provide a renewable source of insulin(+) cells after undergoing cellular reprogramming. Here, we show that cells of the antral stomach have a previously unappreciated propensity for conversion into functional insulin-secreting cells. Native antral endocrine cells share a surprising degree of transcriptional similarity with pancreatic β cells, and expression of β cell reprogramming factors in vivo converts antral cells efficiently into insulin(+) cells with close molecular and functional similarity to β cells. Induced GI insulin(+) cells can suppress hyperglycemia in a diabetic mouse model for at least 6 months and regenerate rapidly after ablation. Reprogramming of antral stomach cells assembled into bioengineered mini-organs in vitro yielded transplantable units that also suppressed hyperglycemia in diabetic mice, highlighting the potential for development of engineered stomach tissues as a renewable source of functional β cells for glycemic control.
Subjects:
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Animals Article Cellular Reprogramming Cellular Reprogramming Techniques Gastric Mucosa Insulin-Secreting Cells Mice
Source:
Cell Stem Cell. 18(3):410-421.
Pubmed ID:
26908146
Pubmed Central ID:
PMC4779391
Document Type:
Journal Article
Funding:
R01DK082889/DK/NIDDK NIH HHS/United States ; R01 DK084056/DK/NIDDK NIH HHS/United States ; U01 DK089536/DK/NIDDK NIH HHS/United States ; R01 DK106253/DK/NIDDK NIH HHS/United States ; P30 HD018655/HD/NICHD NIH HHS/United States ; ... More +
R01DK082889/DK/NIDDK NIH HHS/United States ; R01 DK084056/DK/NIDDK NIH HHS/United States ; U01 DK089536/DK/NIDDK NIH HHS/United States ; R01 DK106253/DK/NIDDK NIH HHS/United States ; P30 HD018655/HD/NICHD NIH HHS/United States ; P30DK034854/DK/NIDDK NIH HHS/United States ; P30 DK034854/DK/NIDDK NIH HHS/United States ; F32 DK107108/DK/NIDDK NIH HHS/United States ; R01 DK082889/DK/NIDDK NIH HHS/United States ; DP-0144-14-00/DP/NCCDPHP CDC HHS/United States ; P30HD18655/HD/NICHD NIH HHS/United States ; R01DK084056/DK/NIDDK NIH HHS/United States ; R00 DK077445/DK/NIDDK NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:62a973d61e0fa52d2d1e9056666eb24ad21bc473536fd33581f431ca265bcbbb
Download URL:
https://stacks.cdc.gov/view/cdc/40277/cdc_40277_DS1.pdf
File Type:

Supporting Files

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