Diversity of the murine antibody response targeting influenza A(H1N1pdm09) hemagglutinin

Details

• Alternative Title:
  Virology
• Personal Author:
  Wilson, Jason R. ; Tzeng, Wen-Pin ; Spesock, April ; Music, Nedzad ; Guo, Zhu ; Barrington, Robert ; Stevens, James ; Donis, Ruben O. ; Katz, Jacqueline M. ; York, Ian A.
• Description:
  Importance ; Protection against influenza viruses is mediated mainly by antibodies, and in most cases this antibody response is narrow, only providing protection against closely-related viruses. In spite of this limited range of protection, recent findings indicate individuals immune to one influenza virus may contain antibodies (generally a minority of the overall response) that are more broadly reactive. These findings have raised the possibility that influenza vaccines could induce a more broadly protective response, reducing the need for frequent vaccine strain changes. However, interpretation of these observations is hampered by the lack of quantitative characterization of the antibody repertoire. In this study, we used single-cell cloning of influenza HA-specific B cells to assess the diversity and nature of the antibody response to influenza hemagglutinin in mice. Our findings help put bounds on the diversity of the anti-hemagglutinin antibody response, as well as characterizing the cross-reactivity, affinity, and molecular nature of the antibody response.
• Subjects:
  • Animals
  • Antibodies, Viral
  • B-Lymphocytes
  • Cloning, Molecular
  • Gene Expression Regulation
  • Genetic Variation
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulins
  More +
• Source:
  Virology. 458-459:114-124
• Pubmed ID:
  24928044
• Pubmed Central ID:
  PMC4904151
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Download URL:
  https://stacks.cdc.gov/view/cdc/39976/cdc_39976_DS1.pdf
• File Type:
  [PDF - 933.02 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c9fb7cb8a1d66ccc310b81a22fbe258216913db5a8abebd3b64f55b2ac3bc942