CDC recommendations for subsequent Zika IgM antibody testing

Details

• Corporate Authors:
  Centers for Disease Control and Prevention (U.S.)
• Description:
  June 21, 2016, 1140 EDT (11:40 AM EDT)
  
  CDCHAN-00392
  
  Testing for Zika virus infection using real-time reverse-transcription polymerase chain reaction (rRT-PCR) molecular assays is now commercially available. When requesting Zika rRT-PCR testing from a commercial laboratory, providers should be aware that commercial laboratories performing rRT-PCR currently do not also offer Zika IgM enzyme-linked immunosorbent assay (ELISA) or confirmatory serologic testing (plaque reduction neutralization test, or PRNT). Therefore, if possible, providers should store a serum aliquot for subsequent Zika IgM ELISA testing if the rRT-PCR assay is negative. Otherwise, collection of an additional serum sample may be necessary.
  
  Recommendations
  
  • rRT-PCR (molecular) testing should be performed for patients possibly exposed to Zika virus who have symptoms consistent with Zika virus infection
  
  • Providers who request molecular testing for Zika virus infection from a commercial testing laboratory are advised to retain and store in a refrigerator (2-8°C) an aliquot of the patient's serum for subsequent Zika IgM ELISA testing if the rRT-PCR is negative
  
  • For specimens that are rRT-PCR negative from the commercial laboratory and no stored serum specimen is available, another serum specimen should be collected within 12 weeks of symptom onset for Zika IgM ELISA testing
  
  • Appropriate samples for molecular testing are serum samples collected <7 days and urine samples collected <14 days after symptom onset. Urine should always be collected with a patient-matched serum specimen.
  
  FINAL HAN 392 CDC Recommendations for Subsequent Zika IgM Antibody Testing_06 21 2016.pdf
  
  
• Subjects:
  Zika Virus Infection/diagnosis
• Source:
  HAN ; 392
• Series:
  Health Alert Network
• Document Type:
  HAN Health Update
• Pages in Document:
  2 unnumbered pages
• Issue:
  392
• Collection(s):
  Health Alert Network (HAN)
• Main Document Checksum:
  urn:sha-512:fbd7847a613ff6929354a576a263ae0a4d45abc3740682101ddf82212ebe2db1a21a89b86821ea8bd9548b2d6aeed7c222e4c2714ba7b844308ca5acf94af4bd
• Download URL:
  https://stacks.cdc.gov/view/cdc/39953/cdc_39953_DS1.pdf
• File Type:
  [PDF - 180.24 KB ]