Welcome to CDC Stacks | Best practices for using PCR to diagnose Haemophilus influenzae and Neisseria meningitidis and identify serotype or serogroup - 39924 | Health Alert Network (HAN)
Stacks Logo
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.
 
 
Help
Clear All Simple Search
Advanced Search
Best practices for using PCR to diagnose Haemophilus influenzae and Neisseria meningitidis and identify serotype or serogroup
  • Published Date:
    June 17, 2016
  • Language:
    English
Filetype[PDF - 59.61 KB]


Details:
  • Corporate Authors:
    Centers for Disease Control and Prevention (U.S.)
  • Series:
    HAN ; 391
  • Keywords:
  • Description:
    June 17, 2016, 13:30 EDT (1:30 PM EDT)

    CDCHAN-00391

    Determining serotype for Haemophilus influenzae (Hi) and serogroup for Neisseria meningitidis (Nm) is crucial for identifying potential outbreaks and determining appropriate public health responses. Several new commercial multiplex polymerase chain reaction (PCR) assays capable of simultaneously testing a single specimen for an array of pathogens that cause blood infections, meningitis, or encephalitis are available. These assays can rapidly identify Hi and Nm species, but most do not determine serotype or serogroup. Laboratories should continue to perform culture and use validated, specific real-time PCR assays capable of detecting and differentiating all six serotypes (a-f) of Hi and six serogroups (A, B, C, W, X, and Y) of Nm; otherwise, additional steps need to be taken including performing a reflex culture or at a minimum retaining a clinical sample for further testing.

    FINAL HAN 391 Best Practices for Using PCR to Diagnose Haemophilus influenzae and Neisseria Mening_06 17 16.pdf

  • Document Type:
    HAN Health Advisory
  • Collection(s):
    Health Alert Network (HAN)
  • Place as Subject:
    United States
  • Supporting Files:
    No Additional Files
No Related Documents.
You May Also Like:
  1. Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
    Personal Author:
    Wu, Henry M; Cordeiro, Soraia M; Harcourt, Brian H; Carvalho, MariadaGloriaS; Azevedo, Jailton; Oliveira, Tainara Q; Leite, Mariela C; Salgado, Katia; Reis, Mitermayer G; Plikaytis, Brian D; Clark, Thomas A; Mayer, Leonard W; Ko, Albert I; Martin, Stacey W; Reis, Joice N;
    Published:
    Jan 22 2013
    Source:
    BMC Infect Dis. 2013; 13:26.
    Description:
    Background : Although cerebrospinal fluid (CSF) culture is the diagnostic reference standard for bacterial meningitis, its sensitivity is limited, particularly when antibiotics were previously administered. CSF Gram staining and real-time PCR are the...
    File Type:
    Filetype[PDF - 275.19 KB]
  2. Laboratory methods for the diagnosis of meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae
    Personal Author:
    Popovic, Tanja; Ajello, Gloria W.; Facklam, Richard R.;
    Corporate Author:
    Centers for Disease Control and Prevention (U.S.); National Center for Infectious Diseases (U.S.); World Health Organization.;
    Published:
    August, 1998
    Description:
    This manual summarizes laboratory techniques used in the isolation and identification of Neisseria meningitidis (the meningococcus), Streptococcus pneumoniae (the pneumococcus) and Haemophilus influenzae from the cerebrospinal fluid and blood of pati...
    File Type:
    Filetype[PDF - 1.32 MB]
  3. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world; Haemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae, Neisseria gonorrhoeae, Salmonella serotype Typhi, Shigella, and Vibrio cholerae
    Personal Author:
    Perilla, Mindy J.
    Corporate Author:
    World Health Organization, Dept. of Communicable Disease Surveillance and Response.; National Center for Infectious Diseases (U.S.);
    Published:
    c2003
    Description:
    I. Introduction -- II. Requirements of a reference laboratory -- -- Bacterial agents of pneumonia and meningitis -- III. Haemophilus influenzae -- IV. Neisseria meningitidis -- V. Streptococcus pneumoniae -- -- Sexually transmitted pathogen for which...
    File Type:
    Filetype[PDF - 1.48 MB]
  4. Bacterial vaccines and serotype replacement: lessons from Haemophilus influenzae and prospects for Streptococcus pneumoniae.
    Personal Author:
    Lipsitch, M.
    Published:
    1999 May-Jun
    Source:
    Emerg Infect Dis. 5(3):336-345.
    Description:
    Conjugate vaccines have reduced the incidence of invasive disease caused by Haemophilus influenzae, type b (Hib), in industrialized countries and may be highly effective against Streptococcus pneumoniae. However, the serotype specificity of these vac...
    File Type:
    Filetype[PDF - 198.75 KB]
  5. Invasive Haemophilus influenzae Serotype e and f Disease, England and Wales
    Personal Author:
    Ladhani, Shamez N.; Collins, Sarah; Vickers, Anna; Litt, David J.; Crawford, Carina; Ramsay, Mary E.; Slack, Mary P.E.;
    Published:
    May 2012
    Source:
    Emerg Infect Dis. 18(5):725-732.
    Description:
    Haemophilus influenzae infection causes serious invasive disease, but incidence of the most virulent serotype, Hib, has dropped since introduction of routine Hib vaccination. In England and Wales, the incidence of 2 other serotypes, Hie and Hif, is i...
    File Type:
    Filetype[PDF - 300.02 KB]