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Best practices for using PCR to diagnose Haemophilus influenzae and Neisseria meningitidis and identify serotype or serogroup
  • Published Date:
    June 17, 2016
  • Series:
    HAN ; 391
  • Language:
Filetype[PDF-59.61 KB]

  • Description:
    June 17, 2016, 13:30 EDT (1:30 PM EDT) CDCHAN-00391 Determining serotype for Haemophilus influenzae (Hi) and serogroup for Neisseria meningitidis (Nm) is crucial for identifying potential outbreaks and determining appropriate public health responses. Several new commercial multiplex polymerase chain reaction (PCR) assays capable of simultaneously testing a single specimen for an array of pathogens that cause blood infections, meningitis, or encephalitis are available. These assays can rapidly identify Hi and Nm species, but most do not determine serotype or serogroup. Laboratories should continue to perform culture and use validated, specific real-time PCR assays capable of detecting and differentiating all six serotypes (a-f) of Hi and six serogroups (A, B, C, W, X, and Y) of Nm; otherwise, additional steps need to be taken including performing a reflex culture or at a minimum retaining a clinical sample for further testing. FINAL HAN 391 Best Practices for Using PCR to Diagnose Haemophilus influenzae and Neisseria Mening_06 17 16.pdf
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